Summary of project PR001658

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001658. The data can be accessed directly via it's Project DOI: 10.21228/M8CX2C This work is supported by NIH grant, U2C- DK119886.


Project ID: PR001658
Project DOI:doi: 10.21228/M8CX2C
Project Title:DRMY1 promotes robust morphogenesis by sustaining translation of a hormone signaling protein
Project Type:MS quantitative analysis
Project Summary:Robustness is the invariant development of phenotype despite environmental changes and genetic perturbations. In the Arabidopsis flower bud, four sepals initiate at robust positions and times and grow to equal size to enclose and protect the inner floral organs. We previously characterized the mutant development related myb-like1 (drmy1), where 3-5 sepals initiate at irregular positions and variable times and grow to different sizes, compromising their protective function. The molecular mechanism underlying this loss of robustness was unclear. Here, we show that drmy1 has reduced TARGET OF RAPAMYCIN (TOR) activity, ribosomal content, and translation. Translation reduction decreases the protein level of ARABIDOPSIS RESPONSE REGULATOR7 (ARR7), a rapidly synthesized and degraded cytokinin signaling inhibitor. The resultant upregulation of cytokinin signaling disrupts the robust positioning of auxin signaling, causing variable sepal initiation. Our work shows that the homeostasis of translation, a ubiquitous cellular process, is crucial for the robust spatiotemporal patterning of organogenesis.
Institute:Cornell University
Department:Plant Biology Section
Laboratory:Roeder Lab
Last Name:Kong
First Name:Shuyao
Address:239 Weill Hall, 526 Campus Road, Ithaca, NY, 14850, USA
Funding Source:Research reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health (NIH) under award numbers R01GM134037 (A.H.K.R.), DP5OD023072 (J.O.B.), and R01GM145814 (J.O.B.); Cornell Graduate School new student fellowship (S.K.); and in part by a Schmittau-Novak Grant from the School of Integrative Plant Science, Cornell University (M.Z.). H.G.G. was supported by NIH Director’s New Innovator Award (DP2 OD024541-01) and NSF CAREER Award (1652236), NIH R01 Award (R01GM139913), and the Koret-UC Berkeley-Tel Aviv University Initiative in Computational Biology and Bioinformatics. H.G.G. is also a Chan Zuckerberg Biohub Investigator.
Contributors:Shuyao Kong, Mingyuan Zhu, M. Regina Scarpin, David Pan, Longfei Jia, Ryan E. Martinez, Simon Alamos, Batthula Vijaya Lakshmi Vadde, Hernan G. Garcia, Shu-Bing Qian, Jacob O. Brunkard, Adrienne H. K. Roeder

Summary of all studies in project PR001658

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
(* : Contains raw data)
ST002571 Quantification of cytokinins in ap1 cal AP1-GR and drmy1 ap1 cal AP1-GR inflorescences using LC-MS Arabidopsis thaliana Cornell University MS 2023-05-11 1 11 Uploaded data (2.3G)*