Summary of project PR001716
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001716. The data can be accessed directly via it's Project DOI: 10.21228/M8WM6F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001716 |
| Project DOI: | doi: 10.21228/M8WM6F |
| Project Title: | Metabolomics Profiling of the Antiproliferative, Anti-migratory and Anti-invasive Potential of Amlodipine in Lung Cancer Cells |
| Project Type: | LC-MS/MS |
| Project Summary: | Lung cancer is still among the most leading causes of cancer-related deaths across the world. Although chemotherapy is considered as a critical choice to manage/limit cancer growth in lung cancer patients with early-stage and advanced cancer stages, it has many limitations including, at least, the severe side effects and chemoresistance. The latter is one of the considerable challenges to lung cancer treatment. Therefore, identification of new alternative therapies with lesser cytotoxic effects when compared to the currently used chemotherapeutics is one of the current research approaches. Calcium channel blockers (CCBs) are emerging as anti-cancer agents in several cancer types. Our objective is to determine the cytotoxic effect of amlodipine on non-small cell lung cancer (NSCLC) cells. Colorimetric MTT cell proliferation assay was used to analyze cell viability following treatments with amlodipine in A549 and H1299 NSCLC cell lines. ANOVA and Tukey’s multiple comparison test were used to detect statistical significance. Half maximal (50%) inhibitory concentration (IC50) values were obtained by applying nonlinear regression curve fit analysis. To assess the effect of amlodipine on A549 and H1299 NSCLC cells migration and invasion scratch wound-healing assay and cell invasion assay were used. Our study revealed that amlodipine significantly reduced proliferation of cancer cells in a dose-dependent fashion with half maximal (50%) inhibitory concentration (IC50) values of 23 and 25.66 µM in A549 and H1299 cells, respectively. Furthermore, amlodipine was able to reduce the invasiveness and migration of cancer cells, both of which are hallmarks in the pathogenesis of cancer, in both cell lines in a dose-dependent manner. Accordingly, our study provides empirical evidence that amlodipine expresses anti-cancer effect to NSCLC cells. However, additional investigations are required to further confirm our results on a larger scale at the clinical level. |
| Institute: | Sharjah Institute for Medical Research |
| Last Name: | Facility |
| First Name: | Core |
| Address: | M32, SIMR, College of Pharmacy, Health Sciences, University of Sharjah |
| Email: | tims-tof@sharjah.ac.ae |
| Phone: | 065057656 |
Summary of all studies in project PR001716
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002755 | Metabolomics Profiling of the Antiproliferative, Anti-migratory and Anti-invasive Potential of Amlodipine in Lung Cancer Cells | Homo sapiens | Sharjah Institute for Medical Research | MS | 2023-07-18 | 1 | 24 | Uploaded data (6.8G)* |
