Summary of project PR001733
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001733. The data can be accessed directly via it's Project DOI: 10.21228/M8PX3J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001733 |
| Project DOI: | doi: 10.21228/M8PX3J |
| Project Title: | Cell Lineage-Guided Microanalytical Mass Spectrometry Reveals Increased Energy Metabolism and Reactive Oxygen Species in the Vertebrate Organizer |
| Project Summary: | We performed targeted metabolomic analysis on the Spemann-Mangold Organizer (SMO) tissue in the frog (Xenopus laevis) and the remainder of dissected embryos (RE). The goal of this study is to quantify a panel of targeted metabolite intermediates from glycolysis, phosphate energy pool, and mitochondrial activity including the TCA cycle. Metabolites were extracted from the dissected tissues, reconstituted, and analyzed using liquid chromatography (LC) electrospray ionization (ESI) mass spectrometry (MS). The targeted metabolite measurements were performed on a trapped ion mobility time-of-flight mass spectrometer (timsTOF PRO, Bruker). Targeted MS assays on the metabolite intermediates produced downstream, complemented by classical fluorescence-based metabolite assays when available, revealed local oxidative stress and enrichment of reactive oxygen species (ROS) in the SMO. |
| Institute: | University of Maryland |
| Department: | Chemistry & Biochemistry |
| Last Name: | Nemes |
| First Name: | Peter |
| Address: | 8051 Regents Drive, College Park, MD 20742, USA |
| Email: | nemes@umd.edu |
| Phone: | 301-405-0373 |
| Funding Source: | National Institutes of Health under Award no. 1R35GM124755 |
| Contributors: | Aparna B. Baxi, Jie Li, Vi M. Quach, and Peter Nemes |
Summary of all studies in project PR001733
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002778 | Cell Lineage-Guided Microanalytical Mass Spectrometry Reveals Increased Energy Metabolism and Reactive Oxygen Species in the Vertebrate Organizer | Xenopus laevis | University of Maryland | MS | 2024-01-10 | 1 | 144 | Uploaded data (1.2G)* |
