Summary of project PR001734

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001734. The data can be accessed directly via it's Project DOI: 10.21228/M8K72B This work is supported by NIH grant, U2C- DK119886.


Project ID: PR001734
Project DOI:doi: 10.21228/M8K72B
Project Title:20-0029 - Role of spatial differentiation of metabolism in local colonization of microbiota along the root axis
Project Summary:Longitudinal gut-axes of animals show spatial heterogeneity of microbiota related to physiological differentiation. Plant roots show functional heterogeneity in cellular architecture, transcriptome, metabolic states, and immunity. We hypothesized that axial differentiation impacts spatial colonization by rhizobiota along the root axis. We developed two growth systems, ArtSoil and CD-Rhizotrons, to dissect Arabidopsis thaliana roots into three segments. We identified distinct rhizobiota communities in the segments, supporting spatial microbiota differentiation along the axis. Root metabolite profiling revealed differential enrichment and specificity. Correlation analyses point to strong reliance of rhizobiota on carbohydrate supply from the host. Bioinformatic analyses and GUS histochemistry indicate sugar and/or microbe-induced accumulation of SWEET2, 4, and 12 sugar uniporters. Profiling of root-segments in sweet mutants showed impaired spatial rhizobiota arrangement. Correlation analysis revealed complex interconnected metabolite-rhizobiota networks. This work uncovers interdependency between root physiology and microbiota colonization and a contribution of SWEETs to shaping local adaptation of root microbiota.
Institute:Heinrich Heine University Düsseldorf
Laboratory:Plant Metabolism and Metabolomics Laboratory
Last Name:Westhoff
First Name:Philipp
Address:Universitätsstr. 1 40225 Düsseldorf
Phone:+49 211-8110926

Summary of all studies in project PR001734

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
(* : Contains raw data)
ST002779 Contribution of sugar transporters to spatial microbiota colonization along the longitudinal root axis of Arabidopsis Arabidopsis thaliana Heinrich Heine University Düsseldorf MS 2024-02-08 1 77 Uploaded data (1.1G)*