Summary of project PR001819
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001819. The data can be accessed directly via it's Project DOI: 10.21228/M8KQ72 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001819 |
| Project DOI: | doi: 10.21228/M8KQ72 |
| Project Title: | O-GlcNAcase activity maintains stress granules for proximity-enhanced ATP production to ensure recovery from stress |
| Project Summary: | Accurate disassembly of stress granules (SGs) after environmental stimuli release is essential for cells to maintain homeostasis , which requires ATP-consuming processes. However, the molecular mechanism whereby regulation of SGs programmatically disassembly and ATP restoration remain poorly understood in mammalian cells. Here we found that defect of OGA in cells leads to aggregates formation, severe autophagy and eventually apoptosis during stress recovery. OGA, which localized in SGs, had no effect on SGs formation but could protect SGs from rapid disassembly during stress recovery stage. Then the SGs localized glycolysis-related enzymes were reserved and concentrated in SGs during stress release for ATP production in a proximity manner, which was vital to guarantee cells resistant to stress and survival during recovery. Finally, supplementation of ATP to OGA knockdown cells during stress recovery significantly rescue cell from aggregates, autophagy and apoptosis. Together, these results describe a brand new mechanism on how OGA regulates the programmed disassembly of stress granules and restoration of ATP to safeguard cell viability in a very precisely programmed process, whose rate is rigorous regulated. |
| Institute: | Zhejiang University |
| Department: | Life Sciences Institute |
| Laboratory: | Shixian Lin |
| Last Name: | Chen |
| First Name: | Yulin |
| Address: | Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang province, China |
| Email: | ychen209@qq.com |
| Phone: | 18868107794 |
Summary of all studies in project PR001819
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002927 | O-GlcNAcase activity maintains stress granules for proximity-enhanced ATP production to ensure recovery from stress | Homo sapiens | Zhejiang University | MS | 2023-10-14 | 1 | 21 | Uploaded data (31.5M)* |
| ST002936 | O-GlcNAcase activity maintains stress granules for proximity-enhanced ATP production to ensure recovery from stress (Part 2) | Homo sapiens | Zhejiang University | MS | 2023-10-19 | 1 | 21 | Uploaded data (14.9M)* |
| ST003174 (Available on 2024-04-30) | O-GlcNAcase activity maintains stress granules for proximity-enhanced ATP production to ensure recovery from stress (Part 3) | Homo sapiens | Zhejiang University | MS | - | - | 12 | Not available |
