Summary of project PR001858
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001858. The data can be accessed directly via it's Project DOI: 10.21228/M8JM83 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001858 |
| Project DOI: | doi: 10.21228/M8JM83 |
| Project Title: | Deciphering the metabolic heterogeneity of hematopoietic stem cells with single-cell resolution |
| Project Type: | Cell Metabolism |
| Project Summary: | Metabolic status is crucial for stem cell functions; however, the metabolic heterogeneity of endogenous stem cells has never been directly assessed. Here, we develop a platform for high-throughput single-cell metabolomics (hi-scMet) of hematopoietic stem cells (HSCs). By combining flow cytometric isolation and nanoparticle-enhanced laser desorption/ionization mass spectrometry, we routinely detected >100 features from single cells. We mapped the single-cell metabolomes of all hematopoietic cell populations, and HSC subpopulations with different division times, detecting 33 features whose levels exhibited trending changes during HSC proliferation. We found progressive activation of oxidative pentose phosphate pathway (OxiPPP) from dormant to active HSCs. Genetic or pharmacological interference with OxiPPP increased reactive oxygen species level in HSCs, reducing HSC self-renewal upon oxidative stress. Together, our work uncovers the metabolic dynamics during HSC proliferation, reveals a role of OxiPPP for HSC activation, and illustrates the utility of hi-scMet in dissecting metabolic heterogeneity of immunophenotypically defined cell populations. |
| Institute: | Shanghai Jiao Tong University |
| Last Name: | CAO |
| First Name: | JING |
| Address: | 1954 Huashan Road, Shanghai, Shanghai, 200030, China |
| Email: | caojing1@sjtu.edu.cn |
| Phone: | 15201957271 |
| Funding Source: | This work was supported by the National Key R&D Program (2022YFE0103500, 2018YFA0107200, 2021YFF0703500, and 2022YFC2502800), Medical-Engineering Joint Funds of Shanghai Jiao Tong University (YG2021ZD09, YG2022QN107, YG2023ZD08), Haihe Laboratory of Cell Ecosystem Innovation Fund (22HHXBSS00016), the National Natural Science Foundation of China (32270785, 31771637, 81730006, 81971771, 22074044, 22122404) and the CAS Youth Interdisciplinary Team (JCTD-2021-12). This work was also sponsored by Shanghai Municipal Science and Technology Major Project Fund, Shanghai Science and Technology Commission (22XD1424000, 22ZR1469000), Shanghai Institutions of Higher Learning (2021-01-07-00-02-E00083), Innovative Research Team of High-Level Local Universities in Shanghai (SHSMU-ZDCX20210700), Innovation Group Project of Shanghai Municipal Health Comission (2019CXJQ03), Innovation Research Plan of Shanghai Municipal Education Commission (ZXWF082101), National Research Center for Translational Medicine Shanghai (TMSK-2021-124, NRCTM(SH)-2021-06), and Fundamental Research Funds for the Central Universities. |
Summary of all studies in project PR001858
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002983 | Deciphering the metabolic heterogeneity of hematopoietic stem cells with single-cell resolution | Mus musculus | Shanghai Jiao Tong University | MS* | 2023-11-28 | 1 | 160 | Uploaded data (381.2M)* |
| ST002986 | Deciphering the metabolic heterogeneity of hematopoietic stem cells with single-cell resolution: Study 2 | Mus musculus | Shanghai Jiao Tong University | MS* | 2023-11-29 | 1 | 1428 | Uploaded data (2.6G)* |
