Summary of project PR001981
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001981. The data can be accessed directly via it's Project DOI: 10.21228/M8NQ82 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001981 |
| Project DOI: | doi: 10.21228/M8NQ82 |
| Project Title: | METTL3-mediated chromatin contacts promote stress granule phase separation through metabolic reprogramming during senescence |
| Project Summary: | METTL3 is the catalytic subunit of the methyltransferase complex, which mediates m6A modification to regulate gene expression. In addition, METTL3 regulates transcription in an enzymatic activity-independent manner by driving changes in high-order chromatin structure. However, how these functions of MTC are coordinated remains unknown. Here we show that the methyltransferase complex coordinates its enzymatic activity-dependent and independent functions to regulate cellular senescence, a state of stable cell growth arrest. Specifically, METTL3-mediated chromatin loops induce Hexokinase 2 expression through the three-dimensional chromatin organization during senescence. Elevated Hexokinase 2 expression subsequently promotes liquid-liquid phase separation, manifesting as stress granule phase separation, by driving metabolic reprogramming. This correlates with an impairment of translation of cell-cycle related mRNAs harboring polymethylated m6A sites. In summary, our results report a coordination of m6A-dependent and -independent function of the methyltransferase complex in regulating senescence through phase separation driven by metabolic reprogramming. |
| Institute: | University of Texas MD Anderson Cancer Center |
| Last Name: | Zhang |
| First Name: | Rugang |
| Address: | 3SCR3.4121, 1901 East RD, Houston, TX, 77054 |
| Email: | rzhang11@mdanderson.org |
| Phone: | 832-748-6422 |
Summary of all studies in project PR001981
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003182 | METTL3-mediated chromatin contacts promote stress granule phase separation through metabolic reprogramming during senescence | Homo sapiens | University of Texas MD Anderson Cancer Center | MS | 2024-05-03 | 1 | 15 | Uploaded data (3.5G)* |
