Summary of project PR002016
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002016. The data can be accessed directly via it's Project DOI: 10.21228/M81C00 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002016 |
| Project DOI: | doi: 10.21228/M81C00 |
| Project Title: | Effects of mitoregulin loss on cardiac and mitochondrial lipids in mice |
| Project Summary: | We and others discovered a highly-conserved mitochondrial transmembrane microprotein, named Mitoregulin (Mtln), that supports lipid metabolism. We reported that Mtln strongly binds cardiolipin (CL), increases mitochondrial respiration and Ca2+ retention capacities, and reduces reactive oxygen species (ROS). Here we extend our observation of Mtln-CL binding and examine Mtln influence on cristae structure and mitochondrial membrane integrity during stress. We demonstrate that mitochondria from constitutive- and inducible Mtln-knockout (KO) mice are susceptible to membrane freeze-damage and that this can be rescued by acute Mtln re-expression. In mitochondrial-simulated lipid monolayers, we show that synthetic Mtln decreases lipid packing and monolayer elasticity. Lipidomics revealed that Mtln-KO heart tissues show broad decreases in 22:6-containing lipids and increased cardiolipin damage/remodeling. Lastly, we demonstrate that Mtln-KO mice suffer worse myocardial ischemia-reperfusion injury, hinting at a translationally-relevant role for Mtln in cardioprotection. Our work supports a model in which Mtln binds cardiolipin and stabilizes mitochondrial membranes to broadly influence diverse mitochondrial functions, including lipid metabolism, while also protecting against stress. |
| Institute: | University of Iowa |
| Last Name: | Boudreau |
| First Name: | Ryan |
| Address: | 4334 PBDB, 169 Newton Rd, Iowa City, IA 52242 |
| Email: | ryan-boudreau@uiowa.edu |
| Phone: | 3193535573 |
Summary of all studies in project PR002016
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003246 | Effects of mitoregulin loss on cardiac and mitochondrial lipids in aged male mice | Mus musculus | University of Iowa | MS | 2024-12-31 | 1 | 10 | Uploaded data (358.5M)* |
| ST003247 | Effects of mitoregulin loss in aged female mice | Mus musculus | University of Iowa | MS | 2024-12-31 | 1 | 10 | Uploaded data (130.6M)* |
| ST003248 | Effects of acute mitoregulin loss on cardiac mitochondrial lipids in mice | Mus musculus | University of Iowa | MS | 2024-12-31 | 1 | 12 | Uploaded data (1.1G)* |
