Summary of project PR002227
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002227. The data can be accessed directly via it's Project DOI: 10.21228/M8RR8B This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002227 |
| Project DOI: | doi: 10.21228/M8RR8B |
| Project Title: | Multi-omic profiling of squamous cell lung cancer identifies metabolites and related genes associated with squamous cell carcinoma |
| Project Summary: | Squamous cell lung carcinoma (SqCC) is the second most common histological subtype of lung cancer. Besides tumor-initiating and promoting DNA, RNA, and epigenetic alterations, aberrant cell metabolism is a hallmark of carcinogenesis. This study aimed to identify SqCC-specific metabolites and key gene regulators that could eventually be used as new anticancer targets. Transcriptional, proteomic, and metabolomic data were gathered for a cohort of resected lung cancers. SqCC-specific differentially expressed genes were integrated with proteogenomic and metabolic data using genome scale metabolic models (GEMs). Findings were validated in cohorts of tumors, normal specimens, and cell lines. In-situ protein expression of SLC6A8 was investigated. Differential gene expression analysis identified a list of 280 strictly SqCC-specific genes. Metabolic profiling identified 7 SqCC-specific metabolites, of which increased creatine and decreased phosphocholine levels matched to SqCC-specific expression of SLC6A8 and CHKA, by matching genes to metabolites through genome scale metabolic models (GEMs) and the Reactome pathways database. Expression of both genes appeared tumor cell-associated, and in particular, the elevated expression of SLC6A8 identified SqCC also in stage IV disease. Elevated creatine levels and overexpression of its transporter SLC6A8 appear a distinct metabolic feature of SqCC. Considering ongoing clinical trials in other malignancies, exploring SLC6A8-inhibition in SqCC appears motivated based on a metabolic addiction hypothesis. |
| Institute: | Lund University |
| Department: | Oncology |
| Laboratory: | Maria Planck and Johan Staaf |
| Last Name: | Arbajian |
| First Name: | Elsa |
| Address: | Medicon Village 404, 22381 Lund, SWEDEN |
| Email: | elsa.arbajian@med.lu.se |
| Phone: | 0046700253200 |
Summary of all studies in project PR002227
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003599 | Multi-omic profiling of squamous cell lung cancer identifies metabolites and related genes associated with squamous cell carcinoma | Homo sapiens | Lund University | MS | 2025-07-25 | 1 | 73 | Uploaded data (7.3G)* |
