Summary of project PR002262
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002262. The data can be accessed directly via it's Project DOI: 10.21228/M87N8N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002262 |
| Project DOI: | doi: 10.21228/M87N8N |
| Project Title: | Multiplatform Metabolomic Profiling of the Unilateral Ureteral Obstruction Murine Model of CKD |
| Project Summary: | In chronic kidney disease (CKD) research, animal models provide invaluable insights into the disease’s etiopathogenesis and progression, particularly through the evaluation of renal tissue. The unilateral ureteral obstruction (UUO) rodent model stands out for its widespread use in CKD studies, due to its advantages to generate renal fibrosis and accelerated mimicry of obstructive nephropathy in humans. Despite its extensive use, the molecular underpinnings driving kidney disease progression remain incompletely understood. Given the crucial interplay between metabolism and fibrosis in CKD, a thorough examination of the UUO renal tissue through metabolomics is required. Untargeted multiplatform analysis enables a comprehensive measurement of the sample metabolic profile, ensuring a maximum coverage of metabolite diversity to yield extensive insights into the metabolism of this renal injury model. Therefore, in this study, murine kidney tissue from the UUO model underwent analysis using three separation techniques—liquid chromatography (LC), gas chromatography (GC), and capillary electrophoresis (CE)—coupled with mass spectrometry (MS). The findings reveal metabolic changes associated with tubulointerstitial fibrosis, impacting essential pathways such as the TCA cycle, urea cycle, polyamine metabolism, amino acids, one-carbon metabolism, purine catabolism, and NAD+ synthesis, among others. Furthermore, fibrosis significantly influences the renal tissue's lipidomic profile, characterized by a general decrease in most lipid classes and an increase in glycerophospholipids with ether substituents, hexosylceramides, and cholesterol esters compared to the control. These results underscore the relevance of the untargeted multiplatform approach to obtain a comprehensive overview of the alterations within the renal metabolic map, paving the way for further exploration of the molecular mechanisms underlying CKD. |
| Institute: | Universidad CEU San Pablo |
| Last Name: | Rupérez |
| First Name: | Francisco |
| Address: | Campus Montepríncipe. Ctra. M-501 km 0 |
| Email: | ruperez@ceu.es |
| Phone: | 913724753 |
Summary of all studies in project PR002262
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003652 | Multiplatform Metabolomic Profiling of the Unilateral Ureteral Obstruction Murine Model of CKD | Mus musculus | Universidad CEU San Pablo | MS* | 2025-04-21 | 1 | 44 | Uploaded data (113.9G)* |
