Summary of project PR002271
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002271. The data can be accessed directly via it's Project DOI: 10.21228/M82V6D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002271 |
| Project DOI: | doi: 10.21228/M82V6D |
| Project Title: | Metabolome trajectory of exercise physiology- a comprehensive study of healthy male and female athletes |
| Project Summary: | BACKGROUND. Integrating metabolomics in sports science provides valuable insights into the biochemistry during physical activity. However, due to their invasiveness, traditional blood sampling methods present challenges in sports settings. The study investigated sex-specific metabolic responses, addressing a significant gap in exercise research, where female participation remains underrepresented. METHODS. To address this, we explored volumetrically accurate microsampling (VAMS) as a dried blood spot (DBS) technique for assessing metabolomic changes in response to acute exercise in more than 130 participants. This study employed a targeted quantitative approach using isotopically-labeled internal standards to measure over 100 metabolites in DBS, providing accurate and traceable results. An accuracy assessment using standard reference material and stability testing over 90 days further evaluated the suitability of DBS for sports metabolomics. RESULTS. Our findings confirm that DBS offers a valid approach to capturing metabolic changes during exercise, reporting a wide panel of metabolites including key metabolites of energy pathways, which correlate well with plasma-derived data but also less studied classes such as pyrimidines. Implementing a straightforward standardization concept established metabolic perturbations upon bout exercise as differences of absolute concentrations. CONCLUSIONS. While metabolic regulations upon exercise are similar in both sexes, differences in the correlation with fitness-related metadata such as peak volitional oxygen consumption (V̇O2peak) and performance, indicate a higher complexity in women and a limitation of previous knowledge to men only. The quantification approach together with the simplicity of the sampling paves the way to expand this type of research towards other fields of personalized medical services. |
| Institute: | University of Vienna |
| Department: | Department of Analytical Chemistry |
| Laboratory: | Koellensperger Lab |
| Last Name: | Schoeny |
| First Name: | Harald |
| Address: | Waehringerstrasse 38 |
| Email: | harald.schoeny@univie.ac.at |
| Phone: | +43 1 4277 52380 |
| Contributors: | Harald Schoeny, Bruno Stelzer, Theresa Hofbauer, Florian Reisenbauer, Yasin El Abiead, Jürgen Scharhag, Gunda Koellensperger |
Summary of all studies in project PR002271
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003661 | Lipidomics facilitates the discovery of diagnostic biomarkers in patients with chronic total occlusion during the perioperative period | Homo sapiens | Zhongshan Hospital Fudan University | MS | 2025-01-20 | 1 | 63 | Uploaded data (64.3M)* |
| ST003662 | Metabolome trajectory of exercise physiology- a comprehensive study of healthy male and female athletes | Homo sapiens | University of Vienna | MS | 2025-05-14 | 1 | 352 | Uploaded data (31.6G)* |
