Summary of project PR002301
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002301. The data can be accessed directly via it's Project DOI: 10.21228/M86G1Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002301 |
| Project DOI: | doi: 10.21228/M86G1Z |
| Project Title: | Reprogramming the metabolome of Centella asiatica (L.) Urban callus: Profiling of newly synthesized cryptic anthocyanins triggered by LED light exposure |
| Project Type: | Plant metabolomics |
| Project Summary: | In addition to the pentacyclic triterpenoid centelloids, Centella asiatica also synthesizes hydroxycinnamic acid conjugates as well as flavonoids. The latter is the major class of secondary plant metabolites and comprises of various subclasses, including anthocyanidins. Anthocyanins are rarely reported in extracts from C. asiatica and differ structurally due to a flavylium (2-phenylchromenylium) ion that carries a positive charge at the oxygen atom of the C-ring of the basic flavonoid structure. Callus of C. asiatica was initiated and propagated on synthetic media and subjected to different light regimes. White callus resulted from white fluorescent illumination, while purple callus developed in response to white light emitting diode (LED) illumination. In order to profile the metabolites responsible for the intense purple colouration, methanolic extracts were prepared from the two cell lines. Total phenolic, flavonoid and anthocyanin content were determined and indicated (i) very low levels of flavonoids and anthocyanins in white callus and (ii) that anthocyanins dominate the flavonoid content of the purple callus. Extracts were subjected to untargeted ultra high-performance liquid chromatography coupled to high-definition mass spectrometry to profile newly synthesised anthocyanins. Metabolite annotation was based on accurate mass determination and characteristic fragmentation patterns. We report on the reprogramming of the metabolome of white C. asiatica callus due to LED illumination and profile the cryptic anthocyanins as well as putative flavonoid and caffeoylquinic acid co-pigments in purple callus. |
| Institute: | University of Johannesburg |
| Department: | Biochemistry |
| Laboratory: | Prof Dubery Lab |
| Last Name: | Hamany Djande |
| First Name: | Claude Yasmine |
| Address: | 81A Fourth Avenue Westdene |
| Email: | claudeh@uj.ac.za |
| Phone: | 0814415123 |
Summary of all studies in project PR002301
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003707 | Reprogramming the metabolome of Centella asiatica (L.) Urban callus: Profiling of newly synthesized cryptic anthocyanins triggered by LED light exposure | Centella asiatica | University of Johannesburg | MS* | 2025-02-24 | 1 | 12 | Uploaded data (300.3M)* |
