Summary of project PR002309
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002309. The data can be accessed directly via it's Project DOI: 10.21228/M85J9C This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002309 |
| Project DOI: | doi: 10.21228/M85J9C |
| Project Title: | CD9-dependent extracellular vesicles mediate the propagation of ferroptosis |
| Project Summary: | Ferroptosis is a form of regulated cell death that is mediated by accumulation of lipid peroxides. Emerging evidence suggests that ferroptosis as a cell-autonomous event can propagate to surrounding cells. Although a detailed mechanistic understanding of is still lacking, such a propagating feature of ferroptosis has far-reaching implications in developmental and pathological processes. Here, we show that the intercellular transmission of ferroptosis relies on an extracellular vesicle (EV)-dependent transport mechanism. Ferroptotic cells produce massive amounts of EVs carrying death signals. Proteomic analysis reveals that these EVs are CD9 positive and contain major components of the mitogen-activated protein kinase (MAPK) signaling. Further untargeted lipidomics results show that EVs secreted from ferroptotic cells (RSL3-EVs) and control EVs (DMSO-EVs) shared similar lipid composition. EV-transported MAP2K1/2 sensitizes target cells for ferroptosis through the action of peroxisome-proliferator activator receptor gamma (PPARG), a transcription factor that controls expression of various components of ferroptosis defense systems. Perturbation of EV-based transport protects adjacent tissue from ferroptosis in vivo. Our study thus uncovers an EV-dependent intercellular transport mechanism of ferroptosis that is mediated by the MAPK-responsive PPARG pathway. |
| Institute: | Zhejiang University |
| Department: | School of Medicine |
| Last Name: | Li |
| First Name: | Yue |
| Address: | 866 Yuhangtang Road, Hangzhou, Zhejiang, 310058, China |
| Email: | liyue9104@zju.edu.cn |
| Phone: | (86)18869960769 |
Summary of all studies in project PR002309
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003720 | CD9-dependent extracellular vesicles mediate the propagation of ferroptosis | Homo sapiens | Zhejiang University | MS | 2026-01-02 | 1 | 12 | Uploaded data (5.5G)* |
