Summary of project PR002318
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002318. The data can be accessed directly via it's Project DOI: 10.21228/M80R8H This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002318 |
| Project DOI: | doi: 10.21228/M80R8H |
| Project Title: | A moderate depletion of cholesterol inhibits CXCL12 mediated direct cell migration in human T cells |
| Project Summary: | Cholesterol is a major component of mammalian cell membranes. It modulates the lipid bilayer properties and also affects the conformations adopted by membrane receptors. Here, we show that moderate cholesterol depletion achieved through the use of cholesterol oxidase (ChOx) increased membrane fluidity and hindered the capacity of T cells to move towards CXCL12 gradients. While fluorescence resonance energy transfer (FRET) studies revealed changes in the conformation of CXCR4, no significant alterations in ligand binding, in other chemokine-mediated signaling pathways, or even in the dynamics of the receptors at the cell membrane were observed. Furthermore, immunocytochemistry approaches indicated that ChOx treatment did not affect the integrity of lipid enriched microdomains containing CXCR4, thereby preserving ligand-mediated receptor nanoclustering. In addition, under these experimental conditions, we detected higher CXCL12-mediated beta-1 integrins activation and increased cell adhesion to fibronectin. Taken together, these findings suggest that by targeting the cell membrane, where these receptors are embedded, it is possible to modulate certain chemokine-mediated functions. |
| Institute: | Centro Nacional De Biotecnologia |
| Last Name: | Mellado |
| First Name: | Mario |
| Address: | C/ Darwin 3, 28049, Madrid, Spain |
| Email: | mmellado@cnb.csic.es |
| Phone: | 915854660 |
Summary of all studies in project PR002318
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003732 | Lipidomic assessment of sphingomyelinase, cholesterol oxidase and cyclodextrin effect in Jurkat Paris | Homo sapiens | Centro Nacional De Biotecnologia | MS | 2026-01-22 | 1 | 18 | Uploaded data (2.2G)* |
