Summary of project PR002320
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002320. The data can be accessed directly via it's Project DOI: 10.21228/M8R83M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002320 |
| Project DOI: | doi: 10.21228/M8R83M |
| Project Title: | CFTR Mutations Regulate Lipid Homeostasis and Inflammatory Signaling in Pancreatic Cancer |
| Project Summary: | CFTR mutations have been implicated in pancreatitis and pancreatic cancer. CFTR is known to affect lipid metabolism and immune regulation. In this study, we employed untargeted lipidomics with iterative tandem mass spectrometry (MS/MS) to characterize the differences in PANC-1 cells with different CFTR states (overexpression, empty vector, and mutations). Our study found that three PANC-1 cells carrying CFTR mutations (p.L88X, p.E681V, p.C1355Y) showed a significantly higher ratio of FA 20:4 (omega-6 PUFA) and FA 22:6 (omega-3 PUFA) compared to the CFTR wild-type (CFTR OE) PANC-1 cells. Among the arachidonoyl species, the compounds PC16:0_20:4 and PC18:0_20:4 showed higher normalized intensities in CFTR-mutated PANC-1 cells compared to CFTR OE. The compound PS18:0_20:4 exhibited a difference only between CFTR p.L88X PANC-1 cells and CFTR OE. The compounds PI16:0_20:4 and PI18:0_20:4 showed differences comparing CFTR p.L88X and p.C1355Y cells with CFTR OE. In the docosahexaenoyl species, the compounds PC18:0_22:6 and PI18:0_22:6 displayed higher normalized intensities in CFTR-mutated PANC-1 cells compared to CFTR OE. The compound PC16:0_22:6 exhibited differences comparing CFTR p.L88X and p.C1355Y cells with CFTR OE. |
| Institute: | Changhai Hospital |
| Last Name: | Jin |
| First Name: | Gang |
| Address: | 168 Changhai Road, Yangpu District, Shanghai, China, 200433 |
| Email: | jingang@smmu.edu.cn |
| Phone: | +86 18621670329 |
Summary of all studies in project PR002320
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003734 | Altered Omega-6/Omega-3 PUFA Ratios and Phospholipid Profiles in CFTR-Mutant PANC-1 Cells Reveal Novel Links Between CFTR Function and Lipid Metabolism | Homo sapiens | Changhai Hospital | MS | 2025-02-19 | 1 | 52 | Uploaded data (1.8G)* |
