Summary of project PR002333
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002333. The data can be accessed directly via it's Project DOI: 10.21228/M82K0C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002333 |
| Project DOI: | doi: 10.21228/M82K0C |
| Project Title: | Phosphatidylinositol remodeling by lysophospholipid acyltransferase 11 ensures embryonic radial glial cell integrity |
| Project Summary: | Arachidonic acid, a vital polyunsaturated fatty acid in brain development, is enriched in phosphatidylinositol (PI). The arachidonic acyl chain in PI is introduced by lysophospholipid acyltransferase 11 (LPLAT11)/membrane-bound O-acyltransferase 7 (MBOAT7), the loss of which causes microcephaly in humans and mice. Here, we show that LPLAT11 deficiency impaired indirect neurogenesis in the developing neocortex, resulting in fewer layer II-V neurons. LPLAT11-deficient radial glial cells had defects in differentiation into intermediate progenitor cells and increased apoptosis. Prior to these anomalies, LPLAT11 deficiency caused a rounding of the Golgi apparatus, accompanied by impaired apical trafficking of E-cadherin, and deregulated apical detachment. Moreover, impaired PI acyl chain remodeling led to a decreased amount of PI(4,5)P2, leading to Golgi apparatus rounding. Thus, these results clarify the underlying mechanism of microcephaly by LPLAT11 deficiency and highlight the critical role of arachidonic acid in PI in the integrity of radial glial cells. |
| Institute: | University of Tokyo |
| Department: | Graduate School of Pharmaceutical Sciences |
| Laboratory: | Department of Health Chemistry |
| Last Name: | Kono |
| First Name: | Nozomu |
| Address: | Hongo 7-3-1,, Bunyo-ku, Tokyo, 113-0033, Japan |
| Email: | nozomu@mol.f.u-tokyo.ac.jp |
| Phone: | 81-3-5841-4723 |
Summary of all studies in project PR002333
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003747 | Phosphatidylinositol remodeling by lysophospholipid acyltransferase 11 ensures embryonic radial glial cell integrity - part 1 | Mus musculus | University of Tokyo | MS | 2025-03-14 | 1 | 52 | Uploaded data (4.2M)* |
| ST003748 | Phosphatidylinositol remodeling by lysophospholipid acyltransferase 11 ensures embryonic radial glial cell integrity - part 2 | Mus musculus | University of Tokyo | MS | 2025-10-28 | 1 | 43 | Uploaded data (3.8M)* |
| ST003749 | Phosphatidylinositol remodeling by lysophospholipid acyltransferase 11 ensures embryonic radial glial cell integrity - part 3 | Mus musculus | University of Tokyo | MS | 2025-03-14 | 1 | 22 | Uploaded data (1.7M)* |
