Summary of project PR002348
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002348. The data can be accessed directly via it's Project DOI: 10.21228/M84C2P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002348 |
| Project DOI: | doi: 10.21228/M84C2P |
| Project Title: | 1H NMR metabolomics applied to detect metabolic markers of early ostedifferentiation of mesenchymal stem cells from multiple donors |
| Project Type: | 1H NMR metabolomics to detect metabolic markers of early ostedifferentiation of human adipose-derived mesenchymal stem cells from multiple donors |
| Project Summary: | Mesenchymal stem cells (MSC) are pivotal bioengineering tools that can be used effectively in tissue regeneration. However, their inherent biological variability due to inter-donor and tissue source heterogeneity often limits therapeutic applications. In addition, the lack of standardized user-independent protocols for MSC handling also contributes to this heterogeneity. Although existing assays are invaluable for detecting well-known gene/protein markers, they are often time-consuming, user-dependent and prone to reproducibility issue. The resulting high variability in MSC behavior and differentiation performance calls for improved characterization guidelines, ideally based on new robust markers. Increasing interest has therefore arisen in the development of new strategies to unambiguously monitor and predict MSC behavior, for an effective selection of donors/cells with superior therapeutic quality. MSC metabolomics appears as a valuable tool, mainly applied to: i) profile the endometabolome of different cell types/origins, ii) identify the effects of donor characteristics, and iii) measure metabolic adaptations to differentiation or other culture conditions. The relevance of metabolomics in stem cell research has been reviewed recently, and both intracellular and extracellular metabolomes have been shown to respond to osteodifferentiation, their articulated interpretation having been attempted. In this study, the intra- and extracellular metabolic features accompanying the osteodifferentiation of hAMSC were characterized for 3 independent donors, over a period of 21 days and compared to proliferating cells. By employing untargeted nuclear magnetic resonance (NMR) metabolomics, it was established a reliable donor-independent metabolic signatures (sets of metabolite variations) that may serve as predictive markers of osteodifferentiation, while elucidating particularly responsive metabolic pathways accompanying the process. The integration of endo- and exometabolomic adaptations should facilitate the development of rapid and non-invasive strategies to detect and predict early MSC osteodifferentiation. |
| Institute: | University of Aveiro |
| Department: | CICECO – Aveiro Institute of Materials, Department of Chemistry |
| Laboratory: | Metabolomics Group |
| Last Name: | Gil |
| First Name: | Ana |
| Address: | CICECO - Departamento de Química, Universidade de Aveiro, Campus de Santiago |
| Email: | agil@ua.pt |
| Phone: | +351234370707 |
Summary of all studies in project PR002348
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003765 | 1H NMR metabolomics applied to detect metabolic markers of early ostedifferentiation of mesenchymal stem cells from multiple donors | Homo sapiens | University of Aveiro | NMR* | 2025-05-19 | 1 | 237 | Uploaded data (321.3M)* |
