Summary of project PR002356
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002356. The data can be accessed directly via it's Project DOI: 10.21228/M83G0N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002356 |
| Project DOI: | doi: 10.21228/M83G0N |
| Project Title: | SCFA biotherapy in humanized gnotobiotic mice by remodels mucosal homeostasis and metabolome |
| Project Type: | Biomedical research |
| Project Summary: | In this experiment human stool donors from a clinical trial of a short chain fatty acid (SCFA)-yielding biotherapy (HAMSAB - high-amylose maize starch) in Type 1 Diabetes (T1D) patients were chosen based on their SCFA response. Individuals with a >two-fold increase in stool butyrate after the biotherapy were classified as ‘responders’ and <two-fold increase were classified as ‘non-responders’. Four participant samples were chosen: 2x non-responders (donor A and B) and 2x responders (donor C and D). The post-biotherapy stool samples from the human donors were colonized into 4 groups of germ-free NOD mice. Colonised mothers mated with germ-free males and the pups studied. Stool collection was from the pups at 7-9 weeks and used for metabolomics. All the mice were NOD/Lt mice (model of type 1 diabetes). The aim for this dataset is to characterize the different microbial metabolites in response to the human donor microbiota from responders (donors C and D) vs non-responders (donors A and B). Age of the pups at stool collection time is not a factor in this study and T1D progression was not monitored because it is too early for its onset. |
| Institute: | University of Queensland |
| Department: | Frazer Institure |
| Laboratory: | Type 1 diabetes pathogenesis laboratory |
| Last Name: | Hamilton-Williams |
| First Name: | Emma |
| Address: | 37 Kent Street, Brisbane Qld 4102 Australia |
| Email: | e.hamiltonwilliams@uq.edu.au |
| Phone: | +61 7 3443 6989 |
| Funding Source: | Juvenile Diabetes Research Foundation (JDRF) Australia (2-SRA-2019-703-M-B); National Health and Medical Research Council (2002917, 2028813); JDRF International (3-SRA-2019-730-S-B); JDRF Australia (201308399) |
| Publications: | SCFA biotherapy delays diabetes in humanized gnotobiotic mice by remodeling mucosal homeostasis and metabolome |
| Contributors: | Tillett BJ, Dwiyanto J, Secombe K, George T, Zhang V, Anderson D, Duggan E, Giri R, Loo D, Stoll T, Morrison M, Begun J, Hill MM, Gurzov EN, Bell K, Saad S, Barlow CK, Creek DJ, Chong CW, Mariño E, Hamilton-Williams EE |
Summary of all studies in project PR002356
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003778 | Human to mouse microbiota transfer model demonstrates disease-modifying effects of the short-chain fatty acid biotherapy modified microbiota | Mus musculus | University of Queensland | MS | 2025-03-27 | 1 | 49 | Uploaded data (7.7G)* |
