Summary of project PR002371
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002371. The data can be accessed directly via it's Project DOI: 10.21228/M8582Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002371 |
| Project DOI: | doi: 10.21228/M8582Z |
| Project Title: | Short-term alterations in dietary amino acids override host genetic susceptibility and reveal mechanisms of Salmonella Typhimurium small intestine colonization |
| Project Type: | Untargeted Metabolomics analysis |
| Project Summary: | In addition to individual genetics, environmental factors, i.e., short-term dietary changes, may influence host susceptibility to gastrointestinal infection. Herein, we developed a model in which CBA/J mice, a genetically resistant strain that tolerates intestinal colonization by the enteric pathogen Salmonella Typhimurium (S. Tm), rapidly succumb to infectious gastroenteritis after exposure to a L-amino acids (AA) rich diet. In mice, S. Tm-gastroenteritis is restricted to the cecum (large intestine), limiting their use to understand S. Tm small intestine (ileum) colonization, a feature of human Salmonellosis. Surprisingly, CBA mice fed AA diet demonstrated enhanced S. Tm ileal colonization and mortality. Using germ-free mice and ileal-fecal slurry transplant, we found that diet-dependent S. Tm ileal colonization to be microbiota-dependent. Mechanistically, S. Tm relied on Fructosyl-asparagine utilization to expand in the ileum during infection. We demonstrate how AA-based diet overrides host genetics by altering the gut microbiota’s ability to prevent S. Tm ileal colonization. |
| Institute: | Vanderbilt University |
| Department: | Chemistry |
| Laboratory: | Center for Innovative Technology |
| Last Name: | CODREANU |
| First Name: | SIMONA Gabriela |
| Address: | 1234 STEVENSON CENTER LANE |
| Email: | SIMONA.CODREANU@VANDERBILT.EDU |
| Phone: | 6158758422 |
Summary of all studies in project PR002371
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003796 | Short-term alterations in dietary amino acids override host genetic susceptibility and reveal mechanisms of Salmonella Typhimurium small intestine colonization | Mus musculus | Vanderbilt University | MS* | 2025-09-17 | 1 | 18 | Uploaded data (13.4G)* |
