Summary of project PR002385
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002385. The data can be accessed directly via it's Project DOI: 10.21228/M8BR77 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002385 |
| Project DOI: | doi: 10.21228/M8BR77 |
| Project Title: | Sulfur Amino Acid Restriction Enhances Exercise Capacity in Mice by Boosting Fat Oxidation in Muscle |
| Project Summary: | Dietary restriction of the sulfur-containing amino acids methionine and cysteine (SAAR) improves body composition, enhances insulin sensitivity, and extends lifespan; benefits seen also with endurance exercise. Yet, the impact of SAAR on skeletal muscle remains largely unexplored. Here we demonstrate that one week of SAAR in sedentary, young, male mice increases endurance exercise capacity. Indirect calorimetry showed that SAAR increased lipid oxidation at rest and delayed the onset of carbohydrate utilization during exercise. Transcriptomic analysis revealed increased expression of genes involved in fatty acid catabolism especially in glycolytic muscle following SAAR. These findings were functionally supported by increased fatty acid circulatory turnover flux and muscle β-oxidation. Reducing lipid uptake from circulation through endothelial cell (EC)-specific CD36 deletion attenuated the running phenotype. Mechanistically, VEGF-signaling inhibition prevented exercise increases following SAAR, without affecting angiogenesis, implicating noncanonical VEGF signaling and EC CD36-dependent fatty acid transport in regulating exercise capacity by influencing muscle substrate availability. |
| Institute: | Princeton University |
| Department: | Department of Chemistry |
| Laboratory: | Prof. Dr. Joshua Rabinowitz |
| Last Name: | Mann |
| First Name: | Charlotte G. |
| Address: | Joseph Stelzmann Strasse 26, 50931 Cologne, Germany |
| Email: | cmann5@uni-koeln.de |
| Phone: | +49 221 478-84102 |
Summary of all studies in project PR002385
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003812 | Sulfur Amino Acid Restriction Enhances Exercise Capacity in Mice by Boosting Fat Oxidation in Muscle - metabolomics from CD36 x PdgfBcre vs WT mice on control or SAAR diet | Mus musculus | Princeton University | MS | 2025-04-18 | 1 | 81 | Uploaded data (2.1G)* |
| ST003813 | Sulfur Amino Acid Restriction Enhances Exercise Capacity in Mice by Boosting Fat Oxidation in Muscle - extensor digitorum longus (EDL) versus soleus (Sol) on Con and SAAR diet metabolomics | Mus musculus | Princeton University | MS | 2025-04-18 | 1 | 27 | Uploaded data (1.2G)* |
| ST003814 | Sulfur Amino Acid Restriction Enhances Exercise Capacity in Mice by Boosting Fat Oxidation in Muscle - extensor digitorum longus (EDL) versus soleus (Sol) on Con and SAAR diet lipidomics | Mus musculus | Princeton University | MS | 2025-04-18 | 1 | 27 | Uploaded data (638.3M)* |
