Summary of project PR002412
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002412. The data can be accessed directly via it's Project DOI: 10.21228/M8VK0H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002412 |
| Project DOI: | doi: 10.21228/M8VK0H |
| Project Title: | Mammary gland metabolism and its relevance to the fetoplacental expression of cytokine signaling in Caveolin-1 null mice |
| Project Summary: | Mice lacking Caveolin-1 (Cav1), a major protein of the lipid raft of plasma membrane, show dysregulated cellular proliferation of mammary gland and an abnormal fetoplacental communication during pregnancy. The aim of this study is to better understand the functional links of mammary gland metabolism with gene expression of the placenta and fetus. Untargeted metabolomics analysis was performed to examine changes in mammary gland metabolism due to the absence of Cav1. The metabolomics analysis detected a total of 141 metabolites. We identified 81 metabolites that showed differential rank order in the level of expression in the mammary glands of Cav1-null compared to control mice. Differential metabolomics analysis identified 11 metabolites that were significantly changed in the mammary gland due to the absence of Cav1. Integrative metabolomics and transcriptomics analyses were applied to untangle functional links of metabolic pathways of the mammary gland with the gene expression changes of the placenta and fetus. The findings of this study show that metabolism and gene expression of the mammary gland are significantly impacted due to the loss of Cav1. Genes associated with specific metabolic and signaling pathways show coordinated expression changed in the placenta, mammary gland and fetal brain in Cav1-null mice. The cytokine signaling pathway emerges as a key player of the molecular crosstalk among the mammary gland, placenta and fetal brain. By interrogating the single-nuclei gene expression data of placenta and fetal brain previously generated from Cav1-null mice, the study further reveals that these metabolic and signaling genes are differentially regulated in specific cell types of the placenta and fetal brain. The findings of this study expand our understanding about the role of mammary gland metabolism in the regulation of fetoplacental communication in mammalian pregnancy. |
| Institute: | University of Missouri-Columbia |
| Last Name: | Behura |
| First Name: | Susanta |
| Address: | 920 East Campus, Columbia, Missouri, 65211, USA |
| Email: | behuras@missouri.edu |
| Phone: | 573-882-1722 |
Summary of all studies in project PR002412
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003855 | Mammary gland metabolism and its relevance to the fetoplacental expression of cytokine signaling in Caveolin-1 null mice | Mus musculus | University of Missouri-Columbia | MS* | 2025-04-11 | 1 | 6 | Uploaded data (22.1M)* |
