Summary of project PR002424
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002424. The data can be accessed directly via it's Project DOI: 10.21228/M89N9N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002424 |
| Project DOI: | doi: 10.21228/M89N9N |
| Project Title: | Commensal Human Gut Microbes Produce Unique Patterns of Neuro-active Compounds |
| Project Type: | Basic Research - Therapeutic Microbes |
| Project Summary: | The human gut microbiota communicates with multiple organs in the human body, including the enteric and central nervous systems, through the production of neuroactive molecules. However, the specific neuroactive compounds produced by commensal gut bacteria remain unclear. In this study, we investigated the capacity of diverse human commensal gut bacteria to produce neurotransmitters, neurotransmitter precursors, and short-chain fatty acids (SCFAs) using targeted and non-targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. We examined eight bacterial species, including the Gram-positive Lactococcus lactis, Enterococcus faecalis, Blautia producta, Clostridium symbiosum, and Streptococcus thermophilus, and the Gram-negative Prevotella copri, Bacteroides fragilis, and Escherichia coli Nissle. Our results demonstrated that these bacteria differentially consumed glutamine, glutamate, and tryptophan, with several species producing neuroactive metabolites. Notably, E. coli Nissle and B. producta produced significant levels of gamma-aminobutyric acid (GABA), while P. copri was the sole producer of tryptamine from tryptophan. Interestingly, none of the tested bacteria produced serotonin or its intermediates, despite consuming tryptophan. In the tyrosine pathway, E. faecalis generated tyramine and dopamine, while L. lactis, B. producta, and E. coli Nissle synthesized L-DOPA. In addition to neurotransmitters, we identified the production of SCFAs by multiple bacterial strains. Of the bacterial species, B. producta exhibited the broadest range of SCFAs produced. These findings suggest that commensal gut microbes have the potential to influence host neurological processes through the production of neuroactive compounds and SCFAs, underscoring the therapeutic possibilities of modulating the gut microbiota to treat neurological disorders. |
| Institute: | Baylor College of Medicine |
| Department: | Pathology & Immunology |
| Laboratory: | Horvath Lab |
| Last Name: | Horvath |
| First Name: | Thomas |
| Address: | 1 Baylor Plaza, Houston, TX, 77030, USA |
| Email: | thomas.horvath2@bcm.edu |
| Phone: | 832-824-0904 |
| Funding Source: | T32GM132055-01, T32DK124191-01A1, K01DK123195, K01DK121869, P30DK123704, P20GM120457, S10OD036416 |
| Publications: | iScience Submission currently under Review |
| Contributors: | Anna M. Tingler1, Charulekha Packirisamy1, Alyssa Guterriez1, Adelaide E. Horvath1,2,3, Makenna Grozis1, Sigmund J. Haidacher4,5, Kathleen M. Hoch4,5, Anthony M. Haag4,5, Numan Oezguen4,5, Kristen A. Engevik1, Amy Engevik1, Thomas D. Horvath4,5*, Melinda A. Engevik1* |
Summary of all studies in project PR002424
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003868 | Commensal Human Gut Microbes Produce Unique Patterns of Neuro-active Compounds | Bacteroides fragilis; Blautia producta; Clostridium symbiosum; Escherichia coli Nissle; Enterococcus faecalis Symbioflor; Lactococcus lactis; Prevotella copri; Streptococcus thermophilus | Medical University of South Carolina | MS | 2025-06-02 | 1 | 27 | Uploaded data (3.1G)* |
| ST003871 | Commensal Human Gut Microbes Produce Unique Patterns of Neuro-active Compounds - Study 2 | Bacteroides fragilis; Blautia producta; Clostridium symbiosum; Escherichia coli Nissle; Enterococcus faecalis Symbioflor; Lactococcus lactis; Prevotella copri; Streptococcus thermophilus | Medical University of South Carolina | MS | 2025-06-02 | 1 | 27 | Uploaded data (4.7G)* |
| ST003873 | Commensal Human Gut Microbes Produce Unique Patterns of Neuro-active Compounds - Study 3 | Bacteroides fragilis; Blautia producta; Clostridium symbiosum; Escherichia coli Nissle; Enterococcus faecalis Symbioflor; Lactococcus lactis; Prevotella copri; Streptococcus thermophilus | Medical University of South Carolina | MS | 2025-06-02 | 1 | 27 | Uploaded data (9G)* |
