Summary of project PR002453
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002453. The data can be accessed directly via it's Project DOI: 10.21228/M8JZ5D This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002453 |
| Project DOI: | doi: 10.21228/M8JZ5D |
| Project Title: | Mitochondrial control of fuel switching through de novo carnitine synthesis |
| Project Summary: | Mitochondrial transport of trimethyllysine is the key first step in de novo biosynthesis of carnitine. In this study, we show that the uncharacterized transporter SLC25A45 mediates this step. Knockout of SLC25A45 leads to impaired fatty acid oxidation due to a decrease in carnitine. To demonstrate this, deuterated trimethyllysine (d9-TML) is traced in vitro (293 cells and primary hepatocytes) as well as subjected to mitochondrial uptake assays. MBP-SLC25A45 in E. coli is used as a non-mammalian system to study trimethyllysine uptake. We further demonstrate a decrease in acylcarnitines via our lipidomic analysis, leading to decreased cold tolerance in vivo. |
| Institute: | BIDMC |
| Last Name: | Auger |
| First Name: | Christopher |
| Address: | 3 Blackfan Street, Boston, MA, 02115, USA |
| Email: | cauger1@bidmc.harvard.edu |
| Phone: | 8579282065 |
Summary of all studies in project PR002453
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003920 | Mitochondrial control of fuel switching through de novo carnitine synthesis | Mus musculus | BIDMC | MS | 2025-12-15 | 1 | 144 | Uploaded data (8.5G)* |
