Summary of project PR002501
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002501. The data can be accessed directly via it's Project DOI: 10.21228/M8C55X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002501 |
| Project DOI: | doi: 10.21228/M8C55X |
| Project Title: | Metabolomics analysis of SNAT2-deficient cells: implications for the discovery of selective transporter inhibitors |
| Project Type: | Manuscript |
| Project Summary: | Amino acid uptake by the solute carrier family of transporter proteins is critical to support cell metabolism, and inhibition of transporter activity represents a tractable strategy to restrict nutrient availability to cancer cells. A small molecule inhibitor of the sodium-coupled neutral amino acid transporter 2 (SNAT2), 3-(N-methyl(4-methylphenyl)sulfonamido)-N-(2-trifluoromethylbenzyl)thiophene-2-carboxamide (MMTC/57E), was recently identified and was shown to inhibit cell proliferation when combined with glucose transport inhibitors in breast and pancreatic cancer cell lines. In this study, we use mass spectrometry-based metabolomics and establish cell-based assays for the SNAT2 transporter. We show that SNAT2 knockout cells have significant defects in amino acid availability. Using our established assays, we fail to observe that MMTC/57E inhibits SNAT2 activity likely due to its poor solubility. |
| Institute: | University of British Columbia |
| Department: | Biochemistry & Molecular Biology |
| Laboratory: | Parker laboratory |
| Last Name: | Parker |
| First Name: | Seth |
| Address: | 950 W 28th Ave, Vancouver, British Columbia, V6H 0B3, Canada |
| Email: | seth.parker@bcchr.ca |
| Phone: | 6048753121 |
Summary of all studies in project PR002501
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003996 | Metabolomics analysis of MMTC/57E or MeAIB treated SNAT2-expressing cells relative to SNAT2-knockout cells or those treated with vehicle. | Mus musculus | University of British Columbia | MS | 2025-07-28 | 1 | 47 | Uploaded data (11.6G)* |
| ST004013 | Aqueous solubility of MMTC/57E in phosphate-buffered saline at room temperature. | University of British Columbia | MS | 2025-07-30 | 1 | 116 | Uploaded data (282.4M)* | |
| ST004014 | Competitive inhibition of SNAT2-dependent MeAIB uptake by saturating concentrations of L-alanine. | Mus musculus | University of British Columbia | MS | 2025-07-28 | 1 | 36 | Uploaded data (241.7M)* |
| ST004016 | Evaluation of SNAT2-dependent and sodium-dependent MeAIB uptake using GCMS | Mus musculus | University of British Columbia | MS | 2025-07-28 | 1 | 54 | Uploaded data (251.6M)* |
| ST004018 | Evaluation of SNAT1-dependent MeAIB uptake using GCMS | Mus musculus | University of British Columbia | MS | 2025-07-28 | 1 | 71 | Uploaded data (313.9M)* |
| ST004022 | Evaluating competitive inhibition of SNAT2-dependent MeAIB uptake by SNAT2 knockout or treatment with L-alanine or 57E. | Mus musculus | University of British Columbia | MS | 2025-07-28 | 1 | 21 | Uploaded data (94.5M)* |
