Summary of project PR002506
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002506. The data can be accessed directly via it's Project DOI: 10.21228/M8QG1M This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002506 |
| Project DOI: | doi: 10.21228/M8QG1M |
| Project Title: | Metabolic and Lipidomic Trade-offs in Helicoverpa armigera: Dynamics Under Plant Protease Inhibitor-Induced Stress |
| Project Type: | Metabolomics |
| Project Summary: | Plant protease inhibitors retard the growth and development of insects by inhibiting their digestive proteases. In response, insects try to adapt to these plant defensive molecules by modulating their protease expression. However, their survival mechanisms might not be limited only to digestive plasticity. To explore this, we performed a comprehensive lipidomics and metabolomics analysis in Helicoverpa armigera fed with a recombinant Capsicum annuum protease inhibitor (rCanPI-7) having unique four inhibitory repeat domains with potent activity against insect trypsins and chymotrypsins. These results revealed that H. armigera employs a dynamic and multifaceted physiological response to dietary stress induced by rCanPI. Upon ingestion of rCanPI-7, down regulation of glycolysis and TCA cycle indicated a decrease in primary energy metabolism while oxidative stress was evident from the depletion of reduced glutathione, peroxidation of membrane lipids, and accumulation of ceramides which are the hallmarks of mitochondrial dysfunction. Investigation of the dynamics in the turnover of different molecules hints that H. armigera activated multiple compensatory strategies such as mobilizing triglycerides and amino acid catabolism as an alternative source of energy, upregulation of antioxidants, membrane remodeling, activation of apoptosis, and shifts in neuromodulatory metabolites linked to cognitive adaptation. Collectively, these findings point to a tightly regulated physiological tug-of-war in H. armigera, where the damaging impact of rCanPI-induced oxidative and nutritional stress is counteracted by a suite of compensatory metabolic, structural, and neuromodulatory adjustments. To our knowledge, this is the first report of lipidomic profiling in H. armigera, providing novel insights into its biochemical resilience and identifying potential metabolic vulnerabilities for enhancing biopesticide strategies. |
| Institute: | Translational Health Science And Technology Institute (THSTI) |
| Department: | NCD |
| Laboratory: | Biomarker lab |
| Last Name: | Kumar |
| First Name: | Yashwant |
| Address: | NCR Biotech Science Cluster,, Faridabad, Haryana, 121001, India |
| Email: | y.kumar@thsti.res.in |
| Phone: | 01292876796 |
Summary of all studies in project PR002506
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004001 | Metabolic and Lipidomic Trade-offs in Helicoverpa armigera: Dynamics Under Plant Protease Inhibitor-Induced Stress | Helicoverpa armigera ( 29058) | Translational Health Science And Technology Institute (THSTI) | MS* | 2025-07-09 | 1 | 18 | Uploaded data (649.3M)* |
| ST004011 | Metabolic and Lipidomic Trade-offs in Helicoverpa armigera: Dynamics Under Plant Protease Inhibitor-Induced Stress-(study 2) | Helicoverpa armigera | Translational Health Science And Technology Institute (THSTI) | MS* | 2025-07-21 | 1 | 18 | Uploaded data (2.1G)* |
