Summary of project PR002515
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002515. The data can be accessed directly via it's Project DOI: 10.21228/M8JN9T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002515 |
| Project DOI: | doi: 10.21228/M8JN9T |
| Project Title: | 13C Metabolic Tracing in Human SGBS Cells Provides a Potential New Approach Methodology for Assessing Metabolism-Disrupting Properties |
| Project Summary: | Due to the increased use and production of plastic materials worldwide, humans are ubiquitously exposed to plastic additives, including plasticizers. Recent research suggests that exposure to certain plasticizers promotes obesity due to their metabolism-disrupting properties. Following the ban on di-(2-ethylhexyl) phthalate (DEHP) and other phthalate plasticizers due to their reproductive toxicity, substitutes such as the plasticizer diisononylcyclohexane-1,2-dicarboxylate (DINCH) have been increasingly used. However, in vitro, studies indicate that the primary metabolite monoisononylcyclohexane-1,2-dicarboxylic acid ester (MINCH) promotes differentiation of human adipocytes. In contrast, no obesogenic effect has been observed in application studies in vivo. The absence of weight-promoting effects of DINCH was confirmed in a recent study with DINCH-exposed C57BL/6N mice, but an increase in adipocyte size in visceral adipose tissue and sex-specific effects on serum lipid levels together with impaired insulin sensitivity were observed. Therefore, as there is still limited information about the potential metabolism-disrupting properties of MINCH, we used 13C tracing as a novel method to investigate the effects of MINCH on the pathway activity of central carbon metabolism in human adipocytes. In contrast to metabolomics, which provides information on changes in the abundance of metabolites, 13C metabolic tracing provides an overview of changes in metabolic pathway activity, enabling an in-depth understanding of how metabolism-disrupting chemicals might disrupt cellular metabolism. The changes in central carbon metabolism activity following MINCH treatment were analyzed after insulin stimulation using three carbon tracers. The project consists of three main studies, depending on the use of the carbon tracer: 1. Analysis of the effects of MINCH on glycolysis and pentose phosphate pathway (PPP) activity, the contribution of glucose to the tricarboxylic acid cycle (TCA) and pyruvate carboxylase-mediated anaplerosis in human preadipocytes and mature adipocytes using [U-13C]glucose; 2. Validation of the effects of MINCH on glycolysis and PPP activity and discrimination of their contribution to glucose metabolism in human preadipocytes and mature adipocytes using [1,2-13C]glucose; 3. Assessment of glyceroneogenesis activity, glutamine contribution to the TCA cycle, oxidative flux through the TCA, reductive carboxylation via isocitrate dehydrogenase (IDH) for lipid synthesis and cycling of metabolites through the TCA cycle in human preadipocytes and mature adipocytes using [U-13C]-glutamine. |
| Institute: | Helmholtz Centre for Environmental Research |
| Last Name: | Engelmann |
| First Name: | Beatrice |
| Address: | Permoserstr. 15 |
| Email: | beatrice.engelmann@ufz.de |
| Phone: | 004934160251099 |
Summary of all studies in project PR002515
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004019 | Analysis of the effects of MINCH on the metabolism of human preadipocytes and mature adipocytes by 13C metabolic tracing with [U-13C]glucose | Homo sapiens | Helmholtz Centre for Environmental Research | MS | 2025-07-24 | 1 | 144 | Uploaded data (141.4M)* |
| ST004020 | Analysis of the effects of MINCH on the metabolism of human preadipocytes and mature adipocytes by 13C metabolic tracing with [1,2-13C]glucose | Homo sapiens | Helmholtz Centre for Environmental Research | MS | 2025-07-24 | 1 | 144 | Uploaded data (130.3M)* |
| ST004021 | Analysis of the effects of MINCH on the metabolism of human preadipocytes and mature adipocytes by 13C metabolic tracing with [U-13C]glutamine | Homo sapiens | Helmholtz Centre for Environmental Research | MS | 2025-07-24 | 1 | 144 | Uploaded data (131.8M)* |
