Summary of project PR002519
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002519. The data can be accessed directly via it's Project DOI: 10.21228/M81N95 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002519 |
| Project DOI: | doi: 10.21228/M81N95 |
| Project Title: | Species-specific serine metabolism differentially controls natural killer cell functions |
| Project Summary: | Immune cells undergo rapid metabolic reprogramming to fuel effector responses. However, whether the metabolic pathways that supply these functions differ between human and mouse immune cells is poorly understood. Using a comparative metabolomics approach, here we show both conserved and species-distinct metabolite alterations in cytokine-activated primary human and mouse NK cells. Activated human NK cells fail to perform de novo serine synthesis, resulting in broadly impaired effector functions when serine starved ex vivo or during in vivo dietary serine restriction, limiting their antitumor function. In contrast, activated mouse NK cells perform de novo serine synthesis to fuel one-carbon metabolism and proliferation, resulting in increased metabolic flexibility during ex vivo and dietary serine restriction. While NK cells from both species require one-carbon metabolism to proliferate and produce interferon-gamma (IFN-γ), GCLC-dependent glutathione synthesis tunes cytotoxic versus inflammatory function in human NK cells. Thus, activated NK cell functions display species-specific requirements for serine metabolism, and environmental serine availability dictates activated human NK cell functions. |
| Institute: | University of California, Los Angeles |
| Department: | MIMG |
| Last Name: | Li |
| First Name: | Joey |
| Address: | 615 Charles E Young Dr S, Los Angeles CA 90095 |
| Email: | joeyhli@g.ucla.edu |
| Phone: | 4086213407 |
Summary of all studies in project PR002519
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004027 | Species-specific serine metabolism differentially controls natural killer cell functions | Homo sapiens; Mus musculus | University of California, Los Angeles | MS | 2025-09-01 | 1 | 12 | Uploaded data (854.2M)* |
