Summary of project PR002539
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002539. The data can be accessed directly via it's Project DOI: 10.21228/M8FR75 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002539 |
| Project DOI: | doi: 10.21228/M8FR75 |
| Project Title: | Comparison of lipidome from phagosomes containing Pam3csk4-beads vs. uncoupled-beads |
| Project Type: | Lipidomics |
| Project Summary: | Goals of the study: Lipids can participate in signaling cascades. LC3-associated phagocytosis (LAP) facilitates cargo elimination during phagocytosis. Lipid signaling during LC3-associated phagocytosis remains understudied. The goal of the study is to evaluate differences in lipid composition at the phagosomal membrane when phagocytes are fed inert beads (biotin labelled) compared with beads labelled with the TLR2 agonist Pam3csk4 that induces LAP. Method: We analyzed isolated phagosomes (~20x10^6/sample) from immortalized bone marrow derived macrophages (iBMDM) fed Pam3csk4-conjugated beads (6 samples) or uncoupled-beads (3 samples). Phagosomes were isolated 1h after phagocytosis stimulation using mechanical disruption of cells and sucrose gradients. Results: The lipid composition differed between the groups with an overall increased in phosphatidylserine species observed in phagosomes containing Pam3csk4-beads. Phosphatidylserine (PS) species were enriched in LAP-induced phagosomes. PS species serve as signaling molecules attracting and docking Rubicon, a key component in the LAP cascade. Limiting PS enrichment at phagosomes using chemical, genetic methods or impeding Rubicon binding to PS blocks LAP progression. Even though LC3-associated phagocytosis has been implicated in disease models such as infectious diseases and cancer, this work is a basic science study not involved directly with diseases or treatments. |
| Institute: | St. Jude Children's Research Hospital |
| Department: | Immunology |
| Laboratory: | Green Lab |
| Last Name: | Palacios |
| First Name: | Gustavo |
| Address: | 262 Danny Thomas Place, Memphis, Tennessee, 38105, USA |
| Email: | Gustavo.Palacios@stjude.org |
| Phone: | 901-595-4448 |
| Funding Source: | NIH |
| Contributors: | Emilio Boada-Romero, Clifford S. Guy, Gustavo Palacios, Luigi Mari, Suresh Poudel, Zhenrui Li, Piyush Sharma, Douglas R. Green* |
Summary of all studies in project PR002539
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004049 | Comparison of lipidome from phagosomes containing Pam3csk4-beads vs. uncoupled-beads | Mus musculus | St Jude Children's Research Hospital | MS | 2025-08-11 | 1 | 20 | Uploaded data (5.3G)* |
