Summary of project PR002544
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002544. The data can be accessed directly via it's Project DOI: 10.21228/M8T269 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002544 |
| Project DOI: | doi: 10.21228/M8T269 |
| Project Title: | Machine learning identifies SLC25A45 as necessary for transport of mitochondrial methylated amino acids and carnitine synthesis |
| Project Summary: | Solute carriers (SLCs) regulate cellular and organismal metabolism by transporting small molecules and ions across membranes, yet the physiological substrates of ~20% remain elusive. To address this, we developed a supervised machine learning platform to predict and prioritize gene-metabolite associations. We uncover SLC25A45 as a mitochondrial transporter linked to serum levels of methylated basic amino acids, products of protein catabolism. Mechanistically, SLC25A45 is necessary for the mitochondrial import of methylated basic amino acids, including ADMA and TML, the latter serving as a precursor for carnitine synthesis. In line with this observation, SLC25A45 loss impairs hepatic carnitine synthesis and upregulation of carnitine-containing metabolites under fasted conditions. By facilitating mitochondrial TML import, SLC25A45 connects protein catabolism to carnitine production, sustaining β-oxidation during fasting. Altogether, our study identifies putative substrates for three SLCs and provides a resource for transporter deorphanization. |
| Institute: | Rockefeller University |
| Last Name: | Artem |
| First Name: | Khan |
| Address: | 1230 York Ave, New York, NY, 10065 |
| Email: | akhan01@rockefeller.edu |
| Phone: | (212) 327-7874 |
Summary of all studies in project PR002544
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004054 | Machine learning identifies SLC25A45 as necessary for transport of mitochondrial methylated amino acids and carnitine synthesis | Homo sapiens | Rockefeller University | MS | 2025-10-10 | 1 | 6 | Uploaded data (457.9M)* |
| ST004060 | Serum profiling of SLC25A45 mice under different diet/fasting conditions | Mus musculus | Rockefeller University | MS | 2025-10-10 | 1 | 55 | Uploaded data (7.1G)* |
