Summary of project PR002567
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002567. The data can be accessed directly via it's Project DOI: 10.21228/M8TV7M This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002567 |
| Project DOI: | doi: 10.21228/M8TV7M |
| Project Title: | Maternal Vitamin D Status Influences Neuroactive Metabolites in Breast Milk and Shapes the Infant Gut Metabolome |
| Project Type: | Basic Research - Nutrition in Pregnancy Study |
| Project Summary: | Human milk contains multiple bioactive components, many of which are influenced by the mother's nutritional status. Previous research has demonstrated that adequate maternal vitamin D levels can influence the protein, lipid, microbial, and immunological profiles of breast milk. However, the impact of maternal vitamin D status on neurotransmitters in breast milk and the infant gut remains largely unexplored. We conducted a post-hoc analysis using breast milk and matched infant stool samples from a 3-month randomized controlled trial where exclusively breastfeeding mothers were examined for vitamin D levels and categorized as sufficient or deficient for vitamin D. Neuroactive metabolites and short-chain fatty acids (SCFAs) were quantified using both targeted and non-targeted liquid chromatography with tandem mass spectrometry (LC-MS/MS). Our findings revealed that breast milk from mothers with sufficient vitamin D levels contained significantly higher concentrations of tryptophan, phenylalanine, and tyrosine compared to milk from mothers with lower vitamin D levels. No significant differences were observed in tryptamine, kynurenine, kynurenic acid, anthranilic acid, quinolinic acid, tyramine, dopamine, epinephrine, or norepinephrine between the two groups. Among SCFAs, only hexanoic acid was significantly elevated in the breast milk of mothers with sufficient vitamin D. A non-targeted metabolomics analysis of infant stool identified distinct metabolite profiles, where oleamide, vaccenic acid, lacto-N-triaose, and N-acetyl-D-glucosamine varied according to maternal vitamin D levels, indicating that maternal nutrient status may influence the infant gut metabolome. These findings suggest that maternal vitamin D status can influence neurotransmitter precursor levels in breast milk and alter the metabolomic profile of infant stool. |
| Institute: | Baylor College of Medicine |
| Department: | Pathology & Immunology |
| Laboratory: | Horvath Lab |
| Last Name: | Horvath |
| First Name: | Thomas |
| Address: | 1 Baylor Plaza, Houston, TX, 77030, USA |
| Email: | thomas.horvath2@bcm.edu |
| Phone: | 832-824-0904 |
| Funding Source: | T32DK124191-01A1, K01DK123195, P30 DK123704, P20 GM120457, NATS NIH KL2TR001452, UL1TR001450, S10OD036416, P30 DK056338 |
| Publications: | American Journal of Physiology - Gastrointestinal & Liver submission is currently under review |
| Contributors: | Alyssa S. Gutierrez1, Katherine E. Chetta2,3, Santosh Thapa4, Sigmund J. Haidacher5,6, Kathleen M. Hoch5,6, John E. Baatz2,3, Anthony M. Haag5,6, Numan Oezguen5,6, Thomas D. Horvath5,6,7, Carol L. Wagner¥2,3, Melinda A. Engevik¥5,8 |
Summary of all studies in project PR002567
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004088 | Maternal Vitamin D Status Influences Neuroactive Metabolites in Breast Milk and Shapes the Infant Gut Metabolome | Homo sapiens | Medical University of South Carolina | MS | 2026-01-02 | 1 | 18 | Uploaded data (4.7G)* |
