Summary of project PR002573
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002573. The data can be accessed directly via it's Project DOI: 10.21228/M82C09 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002573 |
| Project DOI: | doi: 10.21228/M82C09 |
| Project Title: | The loss of Peroxiredoxin 2 in mice disrupts the biochemical aging phenotype in erythrocytes |
| Project Type: | Targeted time course metabolomic profiles of red blood cells in vivo |
| Project Summary: | Biochemical aging is a complex, multi-factorial process involving declines in energy metabolism and the ability to maintain redox states over time. The peroxiredoxin family of enzymes is critical for maintenance of cellular redox states. In mice, enucleate mature red blood cells (RBC) have a lifespan of about 50 days and thus provide an ideal cell to track cellular aging in vivo. In order to characterize the consequence of compromised redox function, we assessed the RBC metabolome at different time points using biotin labeling to isolate specific RBC populations. 183 distinct metabolites were profiled and quantified using mass spectrometry from cell populations of defined ages at approximately 1, 2, and 3 weeks. Interestingly, the general patterns between RBC derived from wildtype and Prdx2 knockout mice RBC were concordant with one another, with significant alterations in ATP producing reactions, as well as redox maintenance and membrane structure maintenance related metabolic reactions. However, most of the age-related metabolite shifts seen in both wildtype and Prdx2 knockout RBC over time were noted to be significantly more pronounced in the Prdx2 knockouts, suggesting an ‘older’ biochemical age phenotype. |
| Institute: | Scripps Research Institute |
| Laboratory: | Jeffrey Friedman |
| Last Name: | Friedman |
| First Name: | Jeffrey |
| Address: | 10550 North Torrey Pines Road La Jolla, CA 92037 |
| Email: | njamshidi@mednet.ucla.edu |
| Phone: | 310.794.1411 |
| Funding Source: | from NIH RO1 DK080232, R21 DK075763 and W8IXWH-10-2-0059 |
| Publications: | submitted |
| Contributors: | Edward D. Karoly, Robert Mohney |
Summary of all studies in project PR002573
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004096 | The loss of Peroxiredoxin 2 in mice leads to a disrupted biochemical aging phenotype in erythrocytes | Mus musculus | Scripps Research Institute | MS | 2025-12-24 | 1 | 48 | Uploaded data (854.6M)* |
