Summary of project PR002627
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002627. The data can be accessed directly via it's Project DOI: 10.21228/M8326S This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002627 |
| Project DOI: | doi: 10.21228/M8326S |
| Project Title: | Targeted Lipidomic Profiling of STBD1 Knockdown in Clear Cell Renal Carcinoma Cells |
| Project Summary: | Clear cell renal cell carcinoma (ccRCC) is characterized by profound metabolic reprogramming, with marked accumulation of glycogen and lipid droplets (LDs). However, the molecular mechanisms linking glycogen metabolism to LD biogenesis remain poorly understood. In this project, we aimed to elucidate the role of the glycogen-binding protein and selective glycophagy receptor STBD1 in coordinating glycogen and lipid droplet metabolism in ccRCC. Using proximity labeling combined with affinity purification mass spectrometry, we identified STBD1 as a novel LD-associated protein. Mechanistic studies demonstrated that STBD1 is targeted to LDs through N-terminal myristoylation, thereby mediating glycogen–LD colocalization and promoting LD biogenesis. Knockdown of STBD1 suppressed LD formation, highlighting a direct metabolic crosstalk between glycogen and lipid metabolism in ccRCC. To further investigate the functional impact of STBD1 loss, we performed proteomic analysis, which revealed significant alterations in proteins related to autophagy and lipid metabolism. Complementary targeted lipidomics analysis quantified 1,684 lipid species across 40 lipid classes. STBD1 knockdown cells exhibited reduced levels of multiple lipid classes, including monoglycerides (MG) and diglycerides (DG), precursors for triglyceride (TG) synthesis. In contrast, phospholipids enriched in polyunsaturated fatty acids (PUFAs) were increased, suggesting a remodeling of lipid composition that may elevate lipid peroxidation potential. In summary, this study uncovers a novel role of STBD1 in bridging glycogen and lipid droplet metabolism, regulating LD biogenesis, and potentially modulating ferroptosis resistance in ccRCC. These findings provide new mechanistic insights into metabolic vulnerabilities of renal cancer and highlight STBD1-related pathways as potential biomarkers and therapeutic targets. |
| Institute: | The Affiliated Cancer Hospital of Zhengzhou University |
| Department: | Medical laboratory |
| Laboratory: | Department of Laboratory Medicine |
| Last Name: | Wang |
| First Name: | Hao |
| Address: | 127 Dongming Road, Jinshui District, Zhengzhou, Henan, China, Zhengzhou, Henan Province, 450003, China |
| Email: | wanghao123@tmu.edu.cn |
| Phone: | +8613642140283 |
Summary of all studies in project PR002627
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004167 | Targeted Lipidomic Profiling of STBD1 Knockdown in Clear Cell Renal Carcinoma Cells | Homo sapiens | The Affiliated Cancer Hospital of Zhengzhou University | MS | 2025-09-25 | 1 | 26 | Uploaded data (1.5G)* |
