Summary of project PR002634
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002634. The data can be accessed directly via it's Project DOI: 10.21228/M85V83 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002634 |
| Project DOI: | doi: 10.21228/M85V83 |
| Project Title: | Directed Rescue Strategy for Enhanced Implant Osteointegration in Aged Rats |
| Project Summary: | Senolytics, which involve the removal of senescent cells from tissues, have emerged as one of the most promising strategies for treating age-related degenerative diseases. In the context of orthopedic treatment, the elimination of senescent cells can also enhance to the osteointegration of implants in elderly patients. However, achieving specific clearance of senescent cells without adversely affecting the function of normal cell remains challenging. To overcome these challenges, we developed a novel implant surface modification technique to achieve specific clearance of locally senescent cells by modulating their metabolism. Our technique also involved modifying implants with BPTES, a glutaminase 1 (GLS1) inhibitor, through π-π stacking with dopamine. This modification effectively induced apoptosis in senescent mesenchymal stem cells (MSCs) through extensive inhibition of GLS1. This effect was attributed to intracellular acidosis resulting from the suppression of glutaminolysis in senescent MSCs. Simultaneously, poly(γ-glutamate) (PGA), modified by a layer-by-layer method, served as a high-density carbon source coating, continuously supporting glutamine metabolism in MSCs without ammonia production. Targeted metabolic analysis revealed that the modified titanium implants significantly altered the metabolic profile of MSCs, enhancing glutamine metabolism, the pentose phosphate pathway (PPP), aerobic glycolysis, and fatty acid oxidation (FAO), which collectively stimulated osteogenic differentiation. In vivo experiments showed that the surface modification significantly reduced the senescence level around implants and promoted osteointegration in aged rats. These findings offer promising insights into the design and application of orthopedic implants for elderly patients. |
| Institute: | Chongqing University |
| Laboratory: | College of Bioengineering, Chongqing University |
| Last Name: | Li |
| First Name: | Xuan |
| Address: | 174 Shazheng St., Shapingba, Chongqing, 400044, China |
| Email: | 201919021061@cqu.edu.cn |
| Phone: | 15310893373 |
| Funding Source: | The National Natural Science Foundation of China (Nos. 52333011, 52021004, 21734002 and 32171327), State Key Project of Research and Development (2022YFB3804400), and the Natural Science Foundation of Chongqing (No. cstc2021jcyj-cxttX0002) |
Summary of all studies in project PR002634
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004175 | The influence of implants on the amino acid metabolism of mesenchymal stem cells (MSCs) | Rattus norvegicus | Chongqing University | MS | 2025-09-11 | 1 | 20 | Uploaded data (3.8M)* |
| ST004176 | The influence of implants on the central carbon metabolism of mesenchymal stem cells | Rattus norvegicus | Chongqing University | MS | 2025-09-11 | 1 | 12 | Uploaded data (24.6M)* |
