Summary of project PR002649
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002649. The data can be accessed directly via it's Project DOI: 10.21228/M87P0Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002649 |
| Project DOI: | doi: 10.21228/M87P0Z |
| Project Title: | In vivo exposure of mixed microplastic particles in mice and its impacts on the murine gut microbiome and metabolome |
| Project Type: | MS quantitative analysis |
| Project Summary: | Microplastics (MPs) are emerging environmental contaminants due to increasing global plastic production and waste. Microplastics, defined as plastic particles less than 5 mm in diameter, are formed through degradation of larger plastics via sunlight, weathering, and microbes. These plastic compounds are widely detected in water, soil, food, as well as human stool and blood. The gut microbiome, often referred to as our second genome, is important in human health and is the primary point of contact for orally ingested microplastics. To investigate the impact of ingested MPs on the gut microbiome and the metabolome, 8-week-old male and female C57/BL6 mice were orally gavaged mixed plastic (5 µm) exposure consisting of polystyrene, polyethylene, and the biodegradable/biocompatible plastic, poly(lactic-co-glycolic acid), twice a week for 4 weeks at 0, 2, or 4 mg/week (n = 8/group). Fecal pellets were collected for bacterial DNA extraction and metagenomic shotgun sequencing, and serum was subjected to targeted and untargeted metabolomics. A total of 1162 bacterial species and 1205 metabolites were evaluated for downstream analysis. MPs exposure resulted in significant sex-specific and dose-dependent changes to the gut microbiome composition along with substantial regulation of predicted metabolic pathways. Untargeted metabolomics in serum showed that a low MPs dose displayed a more prominent effect on key metabolic pathways such as amino acid metabolism, mitochondrial function, and inflammation. Additionally, SCFA-targeted metabolomics showed significant changes in neuroprotective SCFAs levels in both sexes. Our study demonstrates that microplastics dysregulate the gut microbiome and serum metabolome, highlighting potential human disease risks. |
| Institute: | University of Washington |
| Department: | Environmental and Occupational Health Science |
| Laboratory: | Cui Lab |
| Last Name: | Kim |
| First Name: | Kyle |
| Address: | 3150 Stephanie Loop Northeast, Lacey, Washington, 98516, USA |
| Email: | kk1109@uw.edu |
| Phone: | 3606883268 |
| Funding Source: | National Institute of Health (NIH), National Institute of Environmental Health Sciences (NIEHS) |
Summary of all studies in project PR002649
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004200 | untargeted metabolomics in serum of microplastics exposed mice - Untargeted LC/MS | Mus musculus | University of Washington | MS | 2025-10-10 | 1 | 30 | Uploaded data (4.5G)* |
| ST004201 | SCFA quantification of blood serum in microplastic exposed mice - Targeted GC/MS | Mus musculus | University of Washington | MS | 2025-10-10 | 1 | 39 | Uploaded data (1.7G)* |
