Summary of project PR002652
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002652. The data can be accessed directly via it's Project DOI: 10.21228/M8VC26 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002652 |
| Project DOI: | doi: 10.21228/M8VC26 |
| Project Title: | Tumour sampling conditions perturb the metabolic landscape of clear cell renal cell carcinoma |
| Project Summary: | Human isotopic tracer studies are becoming the gold standard for studying cancer metabolism in vivo. Analysed tissues are typically retrieved after surgical resection exposing them to variable amounts of warm ischaemia. Although standardised protocols are emerging, the effects of sampling conditions on the tissue metabolome remain understudied. Here, we perform a 13C-glucose study coupled with metabolomic, transcriptomic, and proteomic profiling in patients with clear cell renal cell carcinoma (ccRCC) to assess the impact of ischaemia on tissues sampled intraoperatively (blood supply intact) and post-surgical resection (tissues exposed to ischaemia). Although several metabolic features were preserved, we demonstrate that ischaemia significantly impacted other metabolic phenotypes of ccRCC, masking key features such as suppressed gluconeogenesis. Notably, kidneys were more metabolically susceptible to ischaemia than these VHL-mutant ccRCC tumours. Despite the overall stability of the proteome and transcription, we also identified subtle degrees of ischaemia-induced perturbations. Using orthotopic ccRCC-derived xenografts, we evidenced that prolonged exposure to ischaemia disrupted the tissue metabolome stability. Overall, minimising tissue ischaemia is pivotal in accurately profiling cancer metabolism in these important and resource-intense patient studies. |
| Institute: | University of Cambridge |
| Last Name: | Yong |
| First Name: | Cissy |
| Address: | Hills Road, CAMBRIDGE, England, CB2 0QQ, United Kingdom |
| Email: | cy295@cam.ac.uk |
| Phone: | +49 221 478- 84308 |
Summary of all studies in project PR002652
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004207 | Tumour sampling conditions perturb the metabolic landscape of clear cell renal cell carcinoma | Homo Sapiens; Mus musculus | University of Cambridge | MS | 2025-09-25 | 1 | 111 | Uploaded data (17.1G)* |
