Summary of project PR002669
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002669. The data can be accessed directly via it's Project DOI: 10.21228/M8NN9R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002669 |
| Project DOI: | doi: 10.21228/M8NN9R |
| Project Title: | Quantitative analysis of metabolomics alterations in H9c2 cardiomyocytes during differentiation |
| Project Summary: | Cardiomyocyte differentiation is a complex process involving significant metabolic remodeling, but its impact on cellular redox state and cell damage remains poorly understood. Using metabolomic, biophysical, and biochemical approaches, we characterized, in vitro, the metabolic shift of differentiating cardiomyocytes and its implications for oxidative damage. We found that differentiating cardiomyocytes undergo a broad metabolic reprogramming from a glycolytic to an oxidative state, marked by increased activity in key pathways, including malate-aspartate shuttle, glutathione metabolism, and tricarboxylic acid cycle. This metabolic transition was associated with mitochondrial enlargement and increased reactive oxygen species (ROS) production. Intriguingly, despite ROS increase, differentiated cells maintained similar levels of DNA damage as cardiomyoblasts and were more resistant to a H₂O₂ challenge. Our findings suggest that metabolic adaptations during cardiomyocyte differentiation enhance their capacity to mitigate oxidative stress damage, providing an adaptive avenue that enables cardiomyocyte survival upon exposure to a rich oxygen environment. |
| Institute: | University of Campinas |
| Last Name: | Amaral |
| First Name: | Alan |
| Address: | Rua Josué de Castro, s/n - Cidade Universitária, Campinas - SP, 13083-970 |
| Email: | a228197@dac.unicamp.br |
| Phone: | +5527996413870 |
Summary of all studies in project PR002669
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004231 | Metabolomic studies of H9c2 cells samples | Rattus norvegicus | University of Campinas | MS | 2025-09-29 | 1 | 15 | Uploaded data (6.7M)* |
