Summary of project PR002716
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002716. The data can be accessed directly via it's Project DOI: 10.21228/M8KK1D This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002716 |
| Project DOI: | doi: 10.21228/M8KK1D |
| Project Title: | Integrated liver and brain lipidomics reveal tissue-specific alcohol effects and systemic intervention of fat-1 gene in mice with alcohol-associated liver disease |
| Project Type: | Original Research |
| Project Summary: | Alcohol-associated liver disease (ALD) involves profound systemic lipid disruption. Using comprehensive 2DLC-MS-based untargeted lipidomics, we characterized the hepatic and cerebral lipidomes in wild-type and fat-1 transgenic (endogenous omega-3 PUFA-producing) mice subjected to a chronic-plus-binge ethanol model. Compared to fat-1 gene, ethanol intake was the dominant factor that induced 228 lipids in the liver and 316 in the brain with significant abundance changes. . Those significantly changed lipids were consistent across wild-type and fat-1 genotypes but highly tissue-specific. Furthermore, fat-1 genotype significantly modulated ethanol's impact and even reversed the regulation of some lipids, including sphingolipids and monosaturated and saturated fatty acyl-containing lipids. Four lipids in the liver and seven lipids in the brain were co-regulated by ethanol, genotype, and their interaction, with excellent discriminatory power in linear discriminant analysis. The odd-chain lipids of those 11 lipids suggest gut microbiome contributions. While ethanol effects were tissue-specific, fat-1 induced consistent responses in the two tissues, indicating conserved protective pathways. Our findings revealed complex lipid network remodeling in ALD through a multi-factorial affecting the liver-brain axis, highlighting the potential of fat-1 gene to mitigate tissue-specific metabolic dysfunction and informing future therapeutic strategies. |
| Institute: | University of Louisville |
| Department: | Chemistry |
| Laboratory: | Dr. Xiang Zhang lab |
| Last Name: | Feng |
| First Name: | Jing |
| Address: | 2310 S Brooks St. Louisville, KY 40208 |
| Email: | jing.feng@louisville.edu |
| Phone: | 5026187846 |
Summary of all studies in project PR002716
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004300 | Two-dimensional liquid chromatography-mass spectrometry (2DLC-MS) platform to conduct an in-depth lipidomic analysis of liver and brain tissues from fat-1 transgenic mice exposed to ethanol. | Mus musculus | University of Louisville | MS | 2025-12-01 | 1 | 28 | Uploaded data (15G)* |
