Summary of project PR002751
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002751. The data can be accessed directly via it's Project DOI: 10.21228/M82G23 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002751 |
| Project DOI: | doi: 10.21228/M82G23 |
| Project Title: | Glutathione-deficiency promotes basal hyperinsulinemia in the insulin secreting cell line INS-1 (832/13) |
| Project Summary: | This study sought to determine the impact of Glutathione (GSH) synthesis on β-cell function. Glutamate-cysteine ligase catalytic subunit (Gclc) is essential for GSH synthesis. To induce GSH deficiency, GCLC was inhibited in INS-1 (832/13) cells using buthionine sulfoximine (BSO). The 24-hour treatment of INS-1 cells with BSO (250 µM) reduced GSH levels to 33% of untreated, however, glucose-stimulated insulin secretion was unchanged. The 24-hour treatment of INS-1 cells with BME-free medium containing BSO further reduced GSH levels to 9% of untreated and elicited significantly higher basal insulin secretion. We sought to determine if higher basal insulin secretion was related to metabolic alteration therefore, we performed a metabolomics screen. This data is presented here. Out of 53 identified metabolites, we found 6 with significantly lower abundance in -BME+BSO as compared to -BME. Those include, adenosine monophosphate, deoxycytidine monophosphate, glucose 1-phosphate, ADP-glucose, thymidine monophostphase, and deoxycytidine diphosphate. |
| Institute: | Yale University |
| Last Name: | Davidson |
| First Name: | Emily |
| Address: | 300 Cedar St, S110, New Haven, CT 06510 |
| Email: | emily.davidson@yale.edu |
| Phone: | 8104797570 |
Summary of all studies in project PR002751
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004339 | Glutathione-deficiency promotes basal hyperinsulinemia in the insulin secreting cell line INS-1 (832/13) | Rattus norvegicus | Yale University | MS | 2026-01-02 | 1 | 19 | Uploaded data (868.1M)* |
