Summary of project PR002774
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002774. The data can be accessed directly via it's Project DOI: 10.21228/M8383Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002774 |
| Project DOI: | doi: 10.21228/M8383Q |
| Project Title: | Convolvulus pluricaulis confers antidepressant and antioxidant effects through conserved metabolic pathways in Drosophila |
| Project Type: | GC-MS |
| Project Summary: | Convolvulus pluricaulis (Shankhpushpi) is a traditional herb used to treat depression and anxiety. Using Drosophila melanogaster as a model, we identified conserved metabolic and molecular pathways mediating its neuroprotective effects. Metabolomic profiling of flies fed C. pluricaulis revealed altered levels of ascorbic acid, glucose, and adenine monophosphate in head tissue. Gene expression analysis showed significant modulation of glut1 (glucose transporter 1), CG6293 (ascorbate transporter), Rdl (resistant to dieldrin), GABA-B-R1 (GABA-B receptor 1), and sod1 (superoxide dismutase 1). Dietary C. pluricaulis reduced depression-like behavior in a stress-induced model and elevated head ascorbate levels. Knockdown of CG6293, sod1, glut1, or GABA-B-R1 abolished the antioxidant effects, and knockdown of CG6293 eliminated the antidepressant effects, implicating these genes as key downstream effectors. Supplementation with L-ascorbic acid mimicked the behavioral and oxidative resilience conferred by C. pluricaulis. Together, these findings reveal conserved antioxidant and anxiolytic mechanisms underlying the pharmacological effects of C. pluricaulis in Drosophila. |
| Institute: | Shiv Nadar Institution of Eminence, Greater Noida, India |
| Department: | Department of Life Sciences |
| Laboratory: | RNA Biology Laboratory |
| Last Name: | Chawla |
| First Name: | Geetanjali |
| Address: | NH91, Tehsil Dadri, Greater Noida, U.P-201314, INDIA |
| Email: | geetanjalichawla25@gmail.com |
| Phone: | +91-9205007117 |
| Funding Source: | DBT-Wellcome Trust India Alliance |
| Publications: | Mitra et al., Convolvulus pluricaulis confers antidepressant and antioxidant effects through conserved metabolic and molecular pathways in Drosophila |
| Contributors: | Geetanjali Chawla |
Summary of all studies in project PR002774
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004376 | Convolvulus pluricaulis confers antidepressant and antioxidant effects through conserved metabolic and molecular pathways in Drosophila | Drosophila melanogaster | Shiv Nadar Institution of Eminence, Greater Noida, India | MS | 2025-11-24 | 1 | 16 | Uploaded data (1.1G)* |
