Summary of project PR002782
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002782. The data can be accessed directly via it's Project DOI: 10.21228/M82C1P This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002782 |
| Project DOI: | doi: 10.21228/M82C1P |
| Project Title: | Longitudinal Multi-omics Profiling Reveals Different Adaptation to Heat Stress in Genomically Divergent Lactating Sows |
| Project Summary: | Heat stress (HS) poses a growing threat to health and productivity across mammals, a problem exacerbated by climate change. Simultaneously, the gut microbiome plays a crucial role in host adaptation to environmental stressors, yet the molecular mechanisms underlying microbiome-mediated heat tolerance remain poorly understood. Although multi-omics profiling has recently emerged as a powerful tool to explore host–microbiome interactions, no prior study, to our knowledge, has simultaneously integrated metagenomics, metatranscriptomics, and metabolomics in genetically characterized lactating mammals under HS conditions. Here, we present a time-resolved, multi-omics analysis of genomically divergent sows (heat-tolerant, TOL, and heat-sensitive, SEN) exposed to controlled HS, with the aim of identifying microbial and metabolic signatures of resilience. Metagenomic analyses revealed enrichment of specific taxa in TOL sows, including Treponema, F23-B02, and Bifidobacterium, with both enduring and time-specific effects. Metatranscriptomic profiling uncovered functional reprogramming in carbohydrate metabolism, membrane remodeling, and oxidative stress responses in TOL animals. These findings were further supported by metabolomic signatures indicating alterations in lipid turnover, amino acid metabolism, and redox homeostasis. Finally, integration of multi-omics data highlighted coordinated, time-specific microbial responses in TOL sows, reflecting robust host–microbiome adaptations to HS. By identifying candidate microbial biomarkers and conserved functional pathways, this study provides new insights into mammalian HS resilience and establishes a framework for cross-species investigations into heat resilience, stress physiology, and microbiome-targeted interventions. |
| Institute: | North Carolina State University |
| Last Name: | Van Vliet |
| First Name: | Stephan |
| Address: | Center for Human Nutrition Studies, Department of Nutrition, Dietetics, and Food Sciences, Utah State University, Logan, UT |
| Email: | stephan.vanvliet@usu.edu |
| Phone: | 1217785001 |
Summary of all studies in project PR002782
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004389 | Longitudinal Multi-omics Profiling Reveals Different Adaptation to Heat Stress in Genomically Divergent Lactating Sows | Sus scrofa domesticus | North Carolina State University | MS | 2025-11-28 | 1 | 109 | Uploaded data (23.6G)* |
