Summary of project PR002806
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002806. The data can be accessed directly via it's Project DOI: 10.21228/M8ZC3H This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002806 |
| Project DOI: | doi: 10.21228/M8ZC3H |
| Project Title: | Profiling the small intestinal microbiota in vitro using 4 donors |
| Project Summary: | The small intestinal microbiota (SIM) has been proven critical for nutrient utilization within the upper gastrointestinal tract. Currently, research is limited due to 1) difficulties accessing this community in vivo and 2) the high level of interindividual variability in taxonomic structure. Here, an in vitro model of the terminal ileum representative of four unique donors was developed and then used to evaluate interindividual variability using Shotgun sequencing and untargeted metabolomics used to examine community function. The results of Shotgun sequencing showed that, in vitro, the communities were representative of their specific inocula and composed of taxa typical of the SIM, such as Klebsiella, Escherichia, Streptococcus, and Enterococcus. Untargeted metabolomics was used to generate metabolic profiles and found that there was a high degree of similarity between the different tested communities in terms of which metabolites were produced. A core set of genes, features, and metabolites were identified by combing the results from sequencing and metabolomics that were shared across all communities. Together, these results found that despite variability in the taxonomic structure of the SIM, there were similarities in functional outcome due to underlying shared gene representation. |
| Institute: | United States Department of Agriculture |
| Department: | Agricultural Research Service |
| Last Name: | Narrowe |
| First Name: | Adrienne |
| Address: | 600 East Mermaid Lane Wyndmoor, PA 19038, Wyndmoor, Pennsylvania, 19038, USA |
| Email: | adrienne.narrowe@usda.gov |
| Phone: | 2158366926 |
Summary of all studies in project PR002806
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004443 | Profiling the small intestinal microbiota in vitro using 4 donors | Human gut microbiome | United States Department of Agriculture | MS* | 2026-01-21 | 1 | 21 | Uploaded data (513.1M)* |
