Summary of project PR002809
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002809. The data can be accessed directly via it's Project DOI: 10.21228/M8K55S This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002809 |
| Project DOI: | doi: 10.21228/M8K55S |
| Project Title: | Lipidomic dataset of mouse optic nerves in a silicone oil–induced ocular hypertension mouse model. |
| Project Summary: | Glaucoma, a leading cause of irreversible blindness, is characterized by progressive retinal ganglion cell (RGC) loss with optic nerve axonal degeneration. Elevated intraocular pressure (IOP) is the major risk factor promoting axon degeneration. Because lipids are integral to axonal membrane composition, and immune signaling modulation, delineating the optic nerve lipidome under IOP elevation conditions is important for defining intraocular pressure-induced molecular remodeling. Profiling the lipidome may uncover lipid pathways amenable to therapeutic targeting for axonal protection and regeneration in glaucomatous optic neuropathy and, potentially, other central nerve systems injuries. We present optic nerve lipidomic datasets generated from a 5-month-old silicone oil–induced ocular hypertension mouse model in C57BL/6J mice with equal numbers of males and females. Unilateral anterior chamber injection of sterile silicone oil (SO) was used to elevate IOP and induce glaucomatous optic nerve injury, while the contralateral eye received an equal volume of balanced salt solution (BSS) and served as the injection control. Optic nerves from SO and BSS eyes were collected at 2 weeks (early injury) and 8 weeks (established optic nerve degeneration) after intraocular injection, snap-frozen, and stored at −80 °C until lipid extraction. Each time point included n = 6 optic nerves per condition. The Bligh and Dyer method was used for lipid extraction, followed by mass spectrometry lipid profiling using reversed phase chromatographic separation performed on Vanquish Horizon ultrahigh performance liquid chromatography (UHPLC) system (Thermo Fisher Scientific, Waltham, MA) coupled to a Q-Exactive Orbitrap Liquid Chromatography Mass Spectrometer (LC/MS-MS). Data was collected in both positive and negative ion modes for each sample. The raw scans were analyzed with LipidSearch 5.1, and the statistical analysis was conducted through Metaboanalyst 6.0. |
| Institute: | University of Miami |
| Department: | Ophthalmology |
| Last Name: | Bhattacharya |
| First Name: | Sanjoy K. |
| Address: | 1638 NW 10th Avenue, Room 706-A, Miami, FL 33136 |
| Email: | sbhattacharya@med.miami.edu |
| Phone: | 3054824103 |
Summary of all studies in project PR002809
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004446 | Lipidomic dataset of mouse optic nerves in a silicone oil–induced ocular hypertension mouse model. | Mus musculus | University of Miami | MS | 2026-01-12 | 1 | 26 | Uploaded data (61.1G)* |
