Summary of project PR002815
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002815. The data can be accessed directly via it's Project DOI: 10.21228/M8SN90 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002815 |
| Project DOI: | doi: 10.21228/M8SN90 |
| Project Title: | Pyruvate Kinase Muscle (PKM)-2 promotes CD4 T cell survival by regulating pyruvate oxidation during homeostasis and expansion |
| Project Summary: | Metabolomics was done on sorted thymic T cell populations. SP4 (CD4+CD8-) cells exhibited the highest relative levels of the pyruvate-derived end product lactate, whereas DN (CD4-CD-) cells showed overall elevated levels of TCA cycle metabolites, indicating a divergence in pyruvate utilization between these subsets. Both SP4 and DN cells also had increased levels of the ancillary metabolites serine and glycine compared to other thymic populations. In contrast, DP (CD4+CD8+) cells, characterized by low PKM2 expression and activity, displayed reduced levels of both glycolytic and TCA intermediates. Interestingly, despite having elevated PKM activity, SP8 (CD4-CD8+) cells mirrored the metabolic profile of DP cells, with similarly diminished glycolytic and TCA metabolite levels. |
| Institute: | National Cancer Institute |
| Department: | Center for Cancer Research |
| Laboratory: | Cancer Innovation Laboratory |
| Last Name: | Subleski |
| First Name: | Jeff |
| Address: | 1050 Boyles Street, Frederick, MD, 21713 |
| Email: | subleskj@mail.nih.gov |
| Phone: | 301-846-1515 |
Summary of all studies in project PR002815
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004455 | Pyruvate Kinase Muscle (PKM)-2 promotes CD4 T cell survival by regulating pyruvate oxidation during homeostasis and expansion | Mus musculus | National Cancer Institute | MS | 2026-01-12 | 1 | 24 | Uploaded data (242.2M)* |
