Summary of project PR002816
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002816. The data can be accessed directly via it's Project DOI: 10.21228/M8NZ73 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002816 |
| Project DOI: | doi: 10.21228/M8NZ73 |
| Project Title: | Deciphering the Metabolomic Landscape of Medicinal Plants in Response to Drought Stress |
| Project Type: | Untargeted Metabolomics |
| Project Summary: | Background: Astragalus membranaceus var. mongholicus is a high-value medicinal plant whose growth and medicinal compound accumulation are severely impacted by drought. Understanding its metabolic adaptation to water deficit is crucial for improving cultivation and quality. While physiological responses are documented, a comprehensive, untargeted metabolomic profile revealing the systemic reprogramming of metabolites across different tissues and drought intensities remains lacking. Objectives: This metabolomics study aimed to comprehensively characterize the drought-induced metabolic responses in A. mongholicus seedlings. Specifically, we sought to: 1) Profile the global metabolite changes in both aboveground and root tissues under light (LD), moderate (MD), and severe (SD) drought stress using liquid chromatography-mass spectrometry (LC-MS); 2) Identify and quantify differentially accumulated metabolites (DAMs) associated with each stress level; 3) Determine the key metabolic pathways perturbed by drought through KEGG enrichment analysis; and 4) Integrate metabolomic findings with physiological data to elucidate the functional role of metabolic changes in drought adaptation. Main Achievements: Untargeted LC-MS metabolomics identified a total of 921 DAMs (562 in positive ion mode, 359 in negative mode) in A. mongholicus seedlings under drought. The number and direction of metabolic changes exhibited clear tissue-specific and stress intensity-dependent patterns. Aboveground tissues responded primarily by down-regulating metabolites, with key up-regulations in the antioxidant metabolite glutathione and its related pathways (glutathione metabolism, cysteine and methionine metabolism) under LD. In roots, an active up-regulation of metabolites dominated, especially under SD. Pathway analysis revealed that roots reconfigured core amino acid metabolism (e.g., tryptophan, tyrosine, arginine, and proline metabolism) to potentially support energy supply and osmotic adjustment. The accumulation of osmolytes like proline and soluble sugars correlated with these metabolic pathway shifts. This study provides the first extensive metabolomic atlas of A. mongholicus under drought stress, uncovering a tissue-divergent strategy: aerial parts enhance antioxidant capacity, while roots rewire primary metabolism for resilience. These findings and the identified metabolic markers offer a valuable resource for understanding and improving drought tolerance in medicinal plants. |
| Institute: | Yili Normal University |
| Department: | College of Biological Science and Technology |
| Last Name: | Kuerban |
| First Name: | Tusong |
| Address: | No. 448, Jiefang West Road (formerly known as Jiefang Road), Yining City |
| Email: | kurban910@163.com |
| Phone: | 0999-8996985 |
Summary of all studies in project PR002816
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004459 | Untargeted Metabolomics Reveals Tissue-Specific Metabolic Reprogramming and Adaptation Strategies in Astragalus membranaceus var. mongholicus Seedlings under Drought Stress | Astragalus membranaceus var. mongholicus | Yili Normal University | MS | 2026-01-06 | 1 | 24 | Uploaded data (474.7M)* |
