Summary of project PR002823
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002823. The data can be accessed directly via it's Project DOI: 10.21228/M8RP0M This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002823 |
| Project DOI: | doi: 10.21228/M8RP0M |
| Project Title: | Sex-specific effects of exogenous asparagine on colorectal tumor growth, 17β-estradiol levels, and aromatase in mice |
| Project Summary: | The study aims to understand the relationship between exogenous asparagine supplementation and 17β-estradiol levels and to elucidate mechanisms underlying sex-dependent signaling during CRC development. We administered asparagine intraperitoneally to tumor-bearing male and female immunodeficient Rag2/Il2RG (R2G2) mice. We performed untargeted metabolomics and found that intraperitoneal asparagine treatment induced sex-dependent intra-tumoral metabolic changes to asparagine, aspartate, glutamine and glutamate levels. To confirm whether asparagine supplementation alters de novo asparagine synthesis in a sex-specific manner, we performed untargeted metabolomics on the tumor xenografts from the R2G2 mice. We examined abundances of metabolites that are involved in the asparagine metabolism pathway including aspartate and glutamine (substrates) and asparagine and glutamate (products). Samples were analyzed in hydrophilic interaction chromatography (HILIC) and reverse phase (RPLC) mass spectrometry (MS) modes. The mass spectrometry data was acquired using a UPLC system (H-Class ACQUITY, Waters Corporation, MA, United States) coupled to a quadrupole time-of flight (QTOF) mass spectrometer (Xevo G2-XS QTOF, Waters Corporation, MA, United States). |
| Institute: | Yale University |
| Department: | Environmental Health Sciences |
| Laboratory: | Caroline Johnson |
| Last Name: | Johnson |
| First Name: | Caroline |
| Address: | 60 College Street, LEPH, Room 509 |
| Email: | caroline.johnson@yale.edu |
| Phone: | 2037852882 |
| Funding Source: | R01 CA289440/CA/NCI NIH HHS/United States |
| Publications: | https://pubmed.ncbi.nlm.nih.gov/40228761/ |
| Contributors: | Oladimeji Aladelokun, Katherine Benitez, Yuying Wang, Abhishek Jain, Domenica Berardi, Georgio Maroun, Xinyi Shen, Jatin Roper, Joanna Gibson, Kaelyn Sumigray, Sajid A Khan, Caroline H Johnson |
Summary of all studies in project PR002823
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004470 | Sex-specific effects of exogenous asparagine on colorectal tumor growth, 17β-estradiol levels, and aromatase in mice | Mus musculus | Yale University | MS* | 2026-01-06 | 1 | 29 | Uploaded data (3.8G)* |
