Summary of project PR002846
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002846. The data can be accessed directly via it's Project DOI: 10.21228/M8SK18 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002846 |
| Project DOI: | doi: 10.21228/M8SK18 |
| Project Title: | Ruminococcoides intestinale Mitigates Ruminococcus gnavus–Associated Allergic Inflammation via Serotonin Metabolic Reprogramming |
| Project Summary: | Host–microbe communication has been understood primarily through immune or metabolic pathways, yet neurotransmitters can also function as bidirectional signals. Here, we identify a serotonin (5-HT)-centered mechanism that integrates microbial competition with mucosal immunity. In the decade-long twin and singleton cohorts, allergic individuals were abundantly colonized by Ruminococcus gnavus, whereas healthy children were enriched in Ruminococcoides intestinale. We demonstrate that R. gnavus engages two complementary pathways: the bacterium expresses the chaperone allergen DnaK to activate pro-allergic IL-33 alarmin signaling, and concurrently enhances its own 5-HT biosynthesis. The combined actions of IL-33 and 5-HT generate a feed-forward loop that amplifies Th2 inflammation and promotes bacterial proliferation. Pharmacological screening further revealed that domperidone, which possesses ancillary 5-HT receptor–blocking activity, effectively inhibits these effects. In contrast, R. intestinale strain Ri-HJH consumes extracellular 5-HT and redirects tryptophan metabolism toward anti-inflammatory indoles, thereby restoring mucosal immune balance. These findings identify 5-HT as a shared metabolite mediating cross-kingdom communication and establish R. intestinale Ri-HJH as a defined probiotic capable of restoring 5-HT homeostasis. |
| Institute: | National Taiwan University Hospital, National Taiwan University |
| Department: | Department of Pediatrics |
| Laboratory: | Yen-Hsuan Ni's lab |
| Last Name: | Huey-Huey |
| First Name: | Chua |
| Address: | No.8, Chung Shan S. Rd.（Zhongshan S. Rd.）, Zhongzheng Dist., Taipei City 10041, Taiwan |
| Email: | hueyhueychua@gmail.com |
| Phone: | +886-2-2312-3456 #71516 |
Summary of all studies in project PR002846
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004517 | Ruminococcoides intestinale Mitigates Ruminococcus gnavus–Associated Allergic Inflammation via Serotonin Metabolic Reprogramming | Homo sapiens | National Taiwan University Hospital, National Taiwan University | MS | 2026-01-12 | 1 | 50 | Uploaded data (1.4G)* |
