Summary of project PR002859
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002859. The data can be accessed directly via it's Project DOI: 10.21228/M83V8G This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR002859 |
| Project DOI: | doi: 10.21228/M83V8G |
| Project Title: | Coculture of Collinsella aerofaciens and Bacteroides thetaiotaomicron under bile acid stress reveals vitamin B6 exchange |
| Project Summary: | Background: Although the bile acid-mediated microbiome-host interaction is known to shape both the composition and functionality of the gut microbiome, the mechanisms by which bile acid stress influences specific microbial metabolic interactions remain poorly understood. To address this gap, we examine the metabolic interplay between two key gut microbes. Bacteroides thetaiotaomicron, one of the most abundant species, possesses a broad enzymatic repertoire for polysaccharide degradation, while Collinsella aerofaciens is associated with liver-related diseases and plays a role in modifying primary bile acids. Results: In anaerobic coculture, C. aerofaciens mitigated the inhibitory effects of deoxycholic acid (DCA) on B. thetaiotaomicron by absorbing DCA from the medium and partially converting it into glycine-conjugated derivatives. Proteomic analysis showed that DCA broadly disrupted amino acid and vitamin metabolism pathways, particularly in B. thetaiotaomicron. In contrast, coculture led to a general upregulation of these pathways in C. aerofaciens, with a marked activation of vitamin B6 metabolism. Additionally, C. aerofaciens exhibited increased production of citrulline and ornithine in coculture. Conclusions: C. aerofaciens alleviates DCA toxicity on B. thetaiotaomicron through absorption, while promoting amino acid and vitamin metabolism, including the vitamin B6 synthesis pathway, during coculture. These results suggest that microbial interactions can enhance resistance to bile acid stress and may influence gut microbiome resilience, with potential relevance for liver- and bile acid–related disorders. |
| Institute: | Helmholtz Centre for Environmental Research |
| Department: | Department of Molecular Toxicology |
| Laboratory: | Functional Metabolomics |
| Last Name: | Wang |
| First Name: | Yan |
| Address: | Permoserstraße 15, 04318, Leipzig, Germany |
| Email: | yan.wang@ufz.de |
| Phone: | +49 341 60251532 |
Summary of all studies in project PR002859
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST004535 | Extracellular and Intracellular DCA derivatives level alteration | Bacteroides thetaiotaomicron,Collinsella aerofaciens | Charles University | MS | 2026-01-19 | 1 | 58 | Uploaded data (388M)* |
| ST004539 | Extracellular short-chain fatty acid level alteration | Bacteroides thetaiotaomicron,Collinsella aerofaciens | Helmholtz Centre for Environmental Research | MS | 2026-01-19 | 1 | 33 | Uploaded data (8.6M) |
| ST004548 | Extracellular amino acid level alteration | Bacteroides thetaiotaomicron,Collinsella aerofaciens | Helmholtz Centre for Environmental Research | MS | 2026-01-21 | 1 | 33 | Uploaded data (4M) |
