Summary of study ST000042

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000041. The data can be accessed directly via it's Project DOI: 10.21228/M8SG64 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000042
Study TitleBALF Control vs ALI by RPLC-MS
Study TypeMS
Study SummaryAcute respiratory distress syndrome (ARDS) remains a significant hazard to human health and is clinically challenging because there are no prognostic biomarkers and no effective pharmacotherapy. The lung compartment metabolome may detail the status of the local environment that could be useful in ARDS biomarker discovery and the identification of drug target opportunities. However, neither the utility of bronchoalveolar lavage fluid (BALF) as a biofluid for metabolomics nor the optimal analytical platform for metabolite identification are established. To address this, we undertook a study to compare metabolites in BALF samples from patients with ARDS and healthy controls using a newly developed liquid chromatography (LC)-mass spectroscopy (MS) platform for untargeted metabolomics. Following initial testing of three different high performance liquid chromatography (HPLC) columns, we determined that reversed phase (RP)-LC and hydrophilic interaction chromatography (HILIC), were the most informative chromatographic methods because they yielded the most and highest quality data. Following confirmation of metabolite identification, statistical analysis resulted in 37 differentiating metabolites in the BALF of ARDS compared with health across both analytical platforms. Pathway analysis revealed networks associated with amino acid metabolism, glycolysis and gluconeogenesis, fatty acid biosynthesis, phospholipids and purine metabolism in the ARDS BALF. The complementary analytical platforms of RPLC and HILIC-LC generated informative, insightful metabolomics data of the ARDS lung environment. Research is published: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4068805/
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2014-03-22
Num Groups2
Total Subjects23
Raw Data AvailableYes
Raw Data File Type(s).stg,.bin,.xml,.cG,.cd
Uploaded File Size7.7 G
Analysis Type DetailLC-MS
Release Date2014-04-21
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8SG64
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000041
Project DOI:doi: 10.21228/M8SG64
Project Title:BALF Studies
Project Type:MS study
Project Summary:Studies of BALF as a biofluid for metabolomics
Institute:University of Michigan
Department:UMich College of Pharmacy
Laboratory:Stringer Lab
Last Name:Stringer
First Name:Kathleen
Address:Ann Arbor, MI
Email:stringek@umich.edu
Phone:734-647-4775
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