Summary of Study ST000097

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000089. The data can be accessed directly via it's Project DOI: 10.21228/M8988G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Show all samples  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST000097
Study TitleNMR analysis of D. melanogaster
Study TypeCold vs Hardy
Study SummaryTwenty replicates of each genetic line (cold hardy and cold tolerant) were collected with 40 flies per replicate.
Institute
University of Florida
DepartmentDepartment of Biochemistry and Molecular Biology
LaboratoryArthur Edison
Last NameChaevien
First NameClendinen
AddressR3-226 Academic Research Building, Department of Biochemistry & Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Emailcsclendinen@ufl.edu
Submit Date2014-08-27
Num Groups2
Total Subjects40
Raw Data AvailableYes
Raw Data File Type(s)fid
Uploaded File Size27 M
Analysis Type DetailNMR
Release Date2015-02-03
Release Version1
Clendinen Chaevien Clendinen Chaevien
https://dx.doi.org/10.21228/M8988G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000089
Project DOI:doi: 10.21228/M8988G
Project Title:13C Metabolomics: Applications at Natural Abundance
Institute:University of Florida
Department:Department of Biochemistry and Molecular Biology
Laboratory:Edison
Last Name:Edison
First Name:Arthur
Address:R3-226 Academic Research Building, Department of Biochemistry & Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Email:aedison@ufl.edu
Phone:352-392-4535

Subject:

Subject ID:SU000116
Subject Type:Insect
Subject Species:Drosophila melanogaster
Taxonomy ID:7227
Gender:Female
Species Group:Insect

Factors:

Subject type: Insect; Subject species: Drosophila melanogaster (Factor headings shown in green)

mb_sample_id local_sample_id Cold Susceptibility
SA005455H2_15Cold Hardy
SA005456H2_14Cold Hardy
SA005457H2_13Cold Hardy
SA005458H2_12Cold Hardy
SA005459H2_16Cold Hardy
SA005460H2_17Cold Hardy
SA005461H2_1Cold Hardy
SA005462H2_19Cold Hardy
SA005463H2_18Cold Hardy
SA005464H2_11Cold Hardy
SA005465H2_20Cold Hardy
SA005466H2_10Cold Hardy
SA005467H2_3Cold Hardy
SA005468H2_2Cold Hardy
SA005469H2_5Cold Hardy
SA005470H2_4Cold Hardy
SA005471H2_6Cold Hardy
SA005472H2_8Cold Hardy
SA005473H2_9Cold Hardy
SA005474H2_7Cold Hardy
SA005475L2_15Cold Susceptible
SA005476L2_14Cold Susceptible
SA005477L2_13Cold Susceptible
SA005478L2_12Cold Susceptible
SA005479L2_20Cold Susceptible
SA005480L2_11Cold Susceptible
SA005481L2_19Cold Susceptible
SA005482L2_18Cold Susceptible
SA005483L2_17Cold Susceptible
SA005484L2_16Cold Susceptible
SA005485L2_6Cold Susceptible
SA005486L2_3Cold Susceptible
SA005487L2_2Cold Susceptible
SA005488L2_1Cold Susceptible
SA005489L2_4Cold Susceptible
SA005490L2_5Cold Susceptible
SA005491L2_9Cold Susceptible
SA005492L2_8Cold Susceptible
SA005493L2_7Cold Susceptible
SA005494L2_10Cold Susceptible
Showing results 1 to 40 of 40

Collection:

Collection ID:CO000099
Collection Summary:Polar-phase metabolites were extracted from homogenized whole fly tissue using an aqueous-optimized metabolite extraction protocol. (Wu, H.; Southam, A. D.; Hines, A.; Viant, M. R. Analytical biochemistry 2008.)
Sample Type:Tissue extract

Treatment:

Treatment ID:TR000117

Sample Preparation:

Sampleprep ID:SP000112
Sampleprep Summary:Twenty replicates of each genetic line (cold hardy and cold tolerant) were collected with 40 flies per replicate. Polar phase extracted metabolites were dried and reconstituted in 40 ?L of 99% D2O and pipetted into 1.5-mm NMR tubes.
Processing Method:Lyophilization
Processing Storage Conditions:-80 degree celsius
Extraction Method:Polar phase extraction

Analysis:

Analysis ID:AN000155
Laboratory Name:Edison
Analysis Type:NMR
Analysis Comments:1D 13C
Software Version:VNMRJ
Operator Name:Chaevien Clendinen
Data Format:Varian
Chromatography ID:CH000111
Num Factors:2
  
Analysis ID:AN000156
Laboratory Name:Edison
Analysis Type:NMR
Analysis Comments:1D 1H
Software Version:VNMRJ
Operator Name:Chaevien Clendinen
Data Format:Varian
Chromatography ID:CH000111
Num Factors:2

NMR:

NMR ID:NM000027
Analysis ID:AN000155
Instrument Name:Varian
Instrument Type:FT-NMR
NMR Experiment Type:1D 13C
Spectrometer Frequency:600 MHz
NMR Probe:1.5 mm HTS cryoprobe
NMR Solvent:D2O
NMR Tube Size:1.5 mm
Shimming Method:Manual
Pulse Sequence:1D 13C
Pulse Width:60
Receiver Gain:28
Temperature:22 degree Celsius
Number Of Scans:8192
Dummy Scans:16
Acquisition Time:0.8s
Relaxation Delay:0.1
Spectral Width:212 ppm
Num Data Points Acquired:51282
Real Data Points:25641
Line Broadening:2 Hz
Zero Filling:2x
Apodization:Exponential
  
NMR ID:NM000028
Analysis ID:AN000156
Instrument Name:Varian
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:600 MHz
NMR Probe:1.5 mm HTS cryoprobe
NMR Solvent:D2O
NMR Tube Size:1.5 mm
Shimming Method:Manual
Pulse Sequence:1D 1H
Water Suppression:Presaturation
Pulse Width:45
Receiver Gain:28
Offset Frequency:4.77
Chemical Shift Ref Cpd:TSP
Temperature:22 degree Celsius
Number Of Scans:64
Dummy Scans:16
Acquisition Time:2.3s
Relaxation Delay:2s
Spectral Width:12 ppm
Num Data Points Acquired:32768
Real Data Points:16384
Zero Filling:2x
Apodization:Cosine squared
  logo