Summary of Study ST000110
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000102. The data can be accessed directly via it's Project DOI: 10.21228/M88C7T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000110 |
| Study Title | SIRM Analysis of human P493 cells under hypoxia in [U-13C/15N] labeled Glutamine medium (Both positive and ion mode FTMS) |
| Study Type | tracer |
| Study Summary | SIRM Analysis of MYC inducible P493 cells under normoxia and hypoxia in [U-13C/15N] labeled Glutamine medium |
| Institute | University of Kentucky |
| Department | Toxicology |
| Last Name | Fan |
| First Name | Teresa |
| twmfan@gmail.com | |
| Submit Date | 2014-09-29 |
| Num Groups | 4 |
| Total Subjects | 8 |
| Study Comments | For protocols and additional information, see Le, A., Lane, A.N., Hamaker, M., Bose, S., Barbi, J., Tsukamoto, T., Rojas, C.J., Slusher, B.S., Zhang, H., Zimmerman. L.J., Liebler, D.C., Slebos, R.J.C., Lorkiewicz, P.K., Higashi, R.M., Fan, T.W-M., and Dang, C.V. Cell Metabolism 15, 110 (2012). |
| Raw Data Available | No |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2015-02-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000102 |
| Project DOI: | doi: 10.21228/M88C7T |
| Project Title: | SIRM Analysis of human P493 |
| Project Summary: | SIRM Analysis of human MYC-inducible P493 cells |
| Institute: | Johns Hopkins University |
| Department: | Pathology |
| Last Name: | Dang |
| First Name: | Chi |
| Email: | cvdang@jhmi.edu |
Subject:
| Subject ID: | SU000129 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | P493 |
| Species Group: | Mammals |
Factors:
Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Oxygen Condition | Treatment |
|---|---|---|---|
| SA006052 | hypox-13C/15N-Gln-plate-1 | hypoxia | None |
| SA006053 | hypox-13C/15N-Gln-plate-2 | hypoxia | None |
| SA006054 | tet-hypox-13C/15N-Gln-plate-1 | hypoxia | Tetracycline |
| SA006055 | tet-hypox-13C/15N-Gln-plate-2 | hypoxia | Tetracycline |
| SA006056 | normox-13C/15N-Gln-plate-2 | normoxia | None |
| SA006057 | normox-13C/15N-Gln-plate-1 | normoxia | None |
| SA006058 | tet-normox-13C/15N-Gln-plate-2 | normoxia | Tetracycline |
| SA006059 | tet-normox-13C/15N-Gln-plate-1 | normoxia | Tetracycline |
| Showing results 1 to 8 of 8 |
Collection:
| Collection ID: | CO000113 |
| Collection Summary: | - |
| Collection Protocol Comments: | Collection and quench of cells in tissue culture |
| Sample Type: | B Cells |
Treatment:
| Treatment ID: | TR000131 |
| Treatment Protocol Comments: | hypox-13C/15N-Gln: cell culture under hypoxia for 24hr + 24hr with [U-13C/15N]-Gln; tet-hypox-13C/15N-Gln: cell culture under hypoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C/15N]-Gln; tet-normox-13C/15N-Gln: cell culture under normoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C/15N]-Gln; normox-13C/15N-Gln: cell culture under normoxia for 24hr + 24hr with [U-13C/15N]-Gln |
Sample Preparation:
| Sampleprep ID: | SP000126 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Comments: | Protein extraction and quantification. |Preparation of polar samples for GCMS and NMR. | Lipid extraction and preparation for FTMS. |
Chromatography:
| Chromatography ID: | CH000124 |
| Chromatography Type: | GC |
Analysis:
| Analysis ID: | AN000183 |
| Laboratory Name: | RCSIRM |
| Analysis Type: | MS |
| Analysis Comments: | FTMS Analytical Experiment with detection of compounds with unique CHONPS molecular formulas. |
| Instrument Name: | Thermo LTQ-FT |
| Chromatography ID: | CH000124 |
| Num Factors: | 4 |
| Num Metabolites: | 3958 |
| Units: | Peak area |
| Analysis ID: | AN000184 |
| Laboratory Name: | RCSIRM |
| Analysis Type: | MS |
| Analysis Comments: | FTMS Analytical Experiment with detection of compounds with unique CHONPS molecular formulas. |
| Instrument Name: | Thermo LTQ-FT |
| Chromatography ID: | CH000124 |
| Num Factors: | 4 |
| Num Metabolites: | 1301 |
| Units: | Peak area |
| Analysis ID: | AN000185 |
| Laboratory Name: | RCSIRM |
| Analysis Type: | MS |
| Analysis Comments: | GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide). |
| Instrument Name: | Thermo ITQ GC-EI-MS |
| Chromatography ID: | CH000124 |
| Num Factors: | 4 |
| Num Metabolites: | 239 |
| Units: | Peak area |
